Annotated protein:Vesicle-associated membrane protein 2 (VAMP-2) (Synaptobrevin-2). Gene symbol: VAMP2. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P63045
antibody wiki:
SynGO gene info:SynGO data @ VAMP2
Ontology domain:Cellular Component
SynGO term:postsynaptic density (GO:0014069)
Synapse type(s):hippocampus
Annotated paper:Hussain S, et al. "Postsynaptic VAMP/Synaptobrevin Facilitates Differential Vesicle Trafficking of GluA1 and GluA2 AMPA Receptor Subunits" PLoS One. 2015 Oct 21;10(10):e0140868 PMID:26488171
Figure(s):Figure 3, 4 and 5
Annotation description:Figure 3A-B: immunoEM shows labelling with the VAMP2 antibody in both the PSD and in scattered postsynaptic vesicles. The small postsynaptic vesicles were located deeply within the postsynaptic cytoplasm or adjacent to the plasma membrane, both at the PSD and lateral plasma membrane. Clusters of a few VAMP2 positive vesicles were often seen at the plasma membrane close to the PSD

Figure 4: confocal images of immunolabeled dissociated rat hippocampal cultures
Figure 4A-C: Immunolabeling showed punctate staining with anti-VAMP2 along neuronal dendrites.
Figure 4D-4F: VAMP2 colocalized with presynaptic vesicle marker, synaptophysin
Figure 4G-4I: VAMP2 colocalized with PSD95

Antibody specificity control using KD in figure 1.
Evidence tracking, Biological System:Cultured neurons
Intact tissue
Evidence tracking, Protein Targeting:Antibody (detection)
Evidence tracking, Experiment Assay:Electron Microscopy
Confocal
Annotator(s):Robin Clement
Guus Smit
Matthijs Verhage
ORCID:0000-0002-2286-1587
0000-0002-2514-0216
Lab:Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands
SynGO annotation ID:4997
Dataset release (version):20231201
View annotation as GO-CAM model:Gene Ontology