| Annotated protein: | Vesicle-associated membrane protein 2 (VAMP-2) (Synaptobrevin-2). Gene symbol: VAMP2. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P63045 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ VAMP2 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic density (GO:0014069) |
| Synapse type(s): | hippocampus |
| Annotated paper: | Hussain S, et al. "Postsynaptic VAMP/Synaptobrevin Facilitates Differential Vesicle Trafficking of GluA1 and GluA2 AMPA Receptor Subunits" PLoS One. 2015 Oct 21;10(10):e0140868 PMID:26488171 |
| Figure(s): | Figure 3, 4 and 5 |
| Annotation description: | Figure 3A-B: immunoEM shows labelling with the VAMP2 antibody in both the PSD and in scattered postsynaptic vesicles. The small postsynaptic vesicles were located deeply within the postsynaptic cytoplasm or adjacent to the plasma membrane, both at the PSD and lateral plasma membrane. Clusters of a few VAMP2 positive vesicles were often seen at the plasma membrane close to the PSD Figure 4: confocal images of immunolabeled dissociated rat hippocampal cultures Figure 4A-C: Immunolabeling showed punctate staining with anti-VAMP2 along neuronal dendrites. Figure 4D-4F: VAMP2 colocalized with presynaptic vesicle marker, synaptophysin Figure 4G-4I: VAMP2 colocalized with PSD95 Antibody specificity control using KD in figure 1. |
| Evidence tracking, Biological System: | Cultured neurons Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Confocal |
| Annotator(s): | Robin Clement Guus Smit Matthijs Verhage |
| ORCID: | 0000-0002-2286-1587 0000-0002-2514-0216 |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 4997 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |