| Annotated protein: | Vesicle-associated membrane protein 2 (VAMP-2) (Synaptobrevin-2). Gene symbol: VAMP2. Taxonomy: Mus musculus (Mouse). Uniprot ID: P63044 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ VAMP2 |
| Ontology domain: | Biological Process |
| SynGO term: | exocytic insertion of neurotransmitter receptor to postsynaptic membrane (GO:0098967) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Jurado S, et al. "LTP requires a unique postsynaptic SNARE fusion machinery" Neuron. 2013 Feb 6;77(3):542-58 PMID:23395379 |
| Figure(s): | Figure 7 |
| Annotation description: | Figure 7: Glycine-induced increase in GluA1 surface expression was absent in cultured neurons prepared from synaptobrevin-2 (VAMP2) knockout mice. Glycine treatment increased the mEPSC amplitude in control cultured neurons but not in cultured neurons lacking synaptobrevin-2, while having no effect on basal mEPSC amplitude. As expected from the presynaptic functions of synpatobrevin-2, the mEPSC frequency was dramatically reduced in the synaptobrevin-2 knockout cultures and was not increased by glycine treatment. Over-expression of wild-type synaptobrevin-2 rescued both the reduction in basal surface GluA1 levels in cultured synaptobrevin-2 knockout neurons as well as the loss of glycine-induced "LTP". Notably, synaptobrevin-2 knockout neurons exhibited an impairment in constitutive TfR recycling, which was rescued by expression of wild-type synaptobrevin-2. Authors conclude: "These findings suggest that synpatobrevin-2 is involved in both the constitutive delivery of AMPARs to the plasma membrane as well as the enhanced regulated delivery of AMPARs during LTP, but is absolutely required only for the latter." |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 4862 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |