| Annotated protein: | Vesicle-associated membrane protein 2 (VAMP-2) (Synaptobrevin-2). Gene symbol: VAMP2. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P63045 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ VAMP2 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of synaptic vesicle membrane (GO:0030285) |
| Synapse type(s): | hippocampus |
| Annotated paper: | Hussain S, et al. "Antibodies raised against aldehyde-fixed antigens improve sensitivity for postembedding electron microscopy" J Neurosci Methods. 2019 Apr 1;317:1-10 PMID:30703389 |
| Figure(s): | Fig 1, Fig 4, Fig 5 |
| Annotation description: | Fig1C and 1D show quantitative immunoEM for VAMP2 located to presynaptic terminals in mice. For controls, preincubation with antigen and VAMP2 KO mice are shown and quantified. Vesicles are difficult to observe here but the antibody is clearly specific. Fig4D-F, 4H: quantitative immunoEM on rat hippocampal cultures, detected by immuno-gold. VAMP2 immune reactivity is most abundant on synaptic vesicles for all 3 antibodies. Fig5D-F: quantitative immunoEM on rat intact tissue, detected by immuno-gold. VAMP2 immune reactivity is most abundant on synaptic vesicles. Given VAMP2 contains a transmembrane domain the annotation "integral component" of synaptic vesicle membrane is selected. |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Jan van Weering (ORCID:0000-0001-5259-4945) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 4167 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |