| Annotated protein: | Vesicle-associated membrane protein 2 (VAMP-2) (Synaptobrevin-2). Gene symbol: VAMP2. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P63045 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ VAMP2 |
| Ontology domain: | Biological Process |
| SynGO term: | synaptic vesicle exocytosis (GO:0016079) |
| Synapse type(s): | cerebral cortex |
| Annotated paper: | Schiavo G, et al. "Tetanus and botulinum-B neurotoxins block neurotransmitter release by proteolytic cleavage of synaptobrevin" Nature. 1992 Oct 29;359(6398):832-5 PMID:1331807 |
| Figure(s): | Fig.1 and Fig.4 |
| Annotation description: | The authors used purified synaptic vesicles isolated from rat cerebral cortex and investigated the effect of treatment with tetanus and botulinum neurotoxins on them. They observed that both tetanus and botulinum neurotoxin B are able to cleave Vamp2 (Fig. 1). Also, they reported that neurotransmitter release is inhibited by injection of tetanus toxin to Aplysia (Fig.4). This inhibition can be delayed by injection of a peptide that contains the cleavage site of tetanus toxin. |
| Evidence tracking, Biological System: | Cultured neurons Cell-free system |
| Evidence tracking, Protein Targeting: | Over-expression Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electrophysiology (generic) Western blot |
| Annotator(s): | Momchil Ninov (ORCID:0000-0002-0808-7003) Mahdokht Kohansalnodehi (ORCID:0000-0002-3898-5197) Reinhard Jahn (ORCID:0000-0003-1542-3498) |
| Lab: | Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, 37077 Göttingen, Germany |
| SynGO annotation ID: | 291 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |