| Annotated protein: | Vesicle-associated membrane protein 2 (VAMP-2) (Synaptobrevin-2). Gene symbol: VAMP2. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P63045 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ VAMP2 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of synaptic vesicle membrane (GO:0030285) |
| Synapse type(s): | brain |
| Annotated paper: | Takamori S, et al. "Molecular anatomy of a trafficking organelle" Cell. 2006 Nov 17;127(4):831-46 PMID:17110340 |
| Figure(s): | Table 1 |
| Annotation description: | In this paper, highly purified synaptic vesicles were isolated from the rat brain. The protein composition of purified vesicles was analyzed by mass spectrometry (MS). Vamp2 was identified as a synaptic vesicle protein. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | none of the above |
| Evidence tracking, Experiment Assay: | Mass-spectrometry Biochemical fractionation (generic) |
| Annotator(s): | Momchil Ninov (ORCID:0000-0002-0808-7003) Mahdokht Kohansalnodehi (ORCID:0000-0002-3898-5197) Reinhard Jahn (ORCID:0000-0003-1542-3498) |
| Lab: | Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, 37077 Göttingen, Germany |
| Additional literature: | Localization of Synaptobrevin (Vamp) was studied for the first time by Baumert et al. They used a combination of subcellular fractionation, Western blotting, and immunogold labeling and showed that synaptobrevin is a synaptic vesicle protein. However, the antibody that they used in this study was not suitable to distinguish between Vamp1 and Vamp2. @ PMID:2498078 In this paper, Edelmann et al. used the specific antibody again Vamp2 to identify the Vamp2 interaction partners by immunoprecipitation. Moreover, performed intact synaptosomes crosslinking. Both experiments revelaed that vamp2 and synaptophysin (synaptic vesicle marker) bind to each other, documenting that vamp2 is a synaptic vesicle protein. @ PMID:7835333 |
| SynGO annotation ID: | 290 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |