| Annotated protein: | Vesicle-associated membrane protein 2 (VAMP-2) (Synaptobrevin-2). Gene symbol: VAMP2. Taxonomy: Mus musculus (Mouse). Uniprot ID: P63044 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ VAMP2 |
| Ontology domain: | Biological Process |
| SynGO term: | synaptic vesicle docking (GO:0016081) |
| Synapse type(s): | hippocampus |
| Annotated paper: | Imig C, et al. "The morphological and molecular nature of synaptic vesicle priming at presynaptic active zones" Neuron. 2014 Oct 22;84(2):416-31 PMID:25374362 |
| Figure(s): | Fig 4, S4, 5A, 5E, 5F; Table 1 |
| Annotation description: | Experiment explanation: The authors systematically characterized the molecular and morphological nature of the docked and primed SV states with nanometer accuracy by the used of high-pressure freezing (HPF), freeze substitution (FS), and electron tomography (ET) of mouse mutants synapses. Taking in account as analysis parameters: SV Diameter and Number of SVs within a distance of: 100, 40, 0-5, 0-2nm of AZ. Protein Function: The analysis of Syb- 2 KO terminals revealed a severe reduction in the number of docked SVs and a prominent accumulation of SVs close (4-8 nm) to the AZ (Figure 4).However, Syb-2 KO synapses showed no changes in the number of SVs within 40 or 100 nm of the AZ. Based on these results, the authors concluded that Syb-2 is required for SV Docking. Protein Location: Confocal microscopy of WT slices showed a specific punctate labeling of Syb-2, which highly colocalized with VGLUT1 but was absent in Syb-2 KO cultures (data not shown). |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Ryan J. Farrell (ORCID:0000-0003-4022-8707) Ghazaleh Ashrafi (ORCID:0000-0001-7480-0826) Camila Pulido (ORCID:0000-0002-5648-066X) Jaime de Juan-Sanz (ORCID:0000-0002-1212-5623) Timothy Ryan (ORCID:0000-0003-2533-9548) |
| Lab: | Department of Biochemistry, Weill Cornell Medicine, New York, NY 10065, USA |
| Additional literature: | Syb-2KO was shown to cause a 90% reduction in the RRP @ PMID:11691998 |
| SynGO annotation ID: | 265 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |