| Annotated protein: | Neurofilament medium polypeptide (NF-M) (160 kDa neurofilament protein) (Neurofilament 3) (Neurofilament triplet M protein). Gene symbol: NEFM. Taxonomy: Mus musculus (Mouse). Uniprot ID: P08553 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ NEFM |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic intermediate filament cytoskeleton (GO:0099160) |
| Synapse type(s): | striatum neocortex |
| Annotated paper: | Yuan A, et al. "Neurofilament subunits are integral components of synapses and modulate neurotransmission and behavior in vivo" Mol Psychiatry. 2015 Aug;20(8):986-94 PMID:25869803 |
| Figure(s): | Fig1, FigS2 and Fig2 |
| Annotation description: | Fig1 shows by quantitative immunoEM that NFM (neurofilament medium) is mainly present in the postsynapse with also significant labelling in the presynapse compared to background labeling in mitochondria (Fig1b). Fig1A, C and D show typical examples. Similar finding are reported using fluorescence microscopy where NFM colocalises with postsynaptic marker PSD95 (Fig1g) and presynaptic marker synaptophysin (Fig1h). FigS2E shows NFM labelling by pre-embedding methods, which is quantified against the neurofilament triple knockout (FigS2i) showing that the antibody is specific. |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Wide-field fluorescence |
| Annotator(s): | Jan van Weering (ORCID:0000-0001-5259-4945) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 997 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |