| Annotated protein: | Src substrate cortactin. Gene symbol: CTTN. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q66HL2 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CTTN |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic actin cytoskeleton (GO:0098871) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Racz B, et al. "The subcellular organization of cortactin in hippocampus" J Neurosci. 2004 Nov 17;24(46):10310-7 PMID:15548644 |
| Figure(s): | Fig 3, Fig 4b-e, Fig 5b-e, Fig 6a,b, Table 1 |
| Annotation description: | Authors investigate the cellular localization of the actin-binding protein cortactin in rat brain using confocal imaging and immuno EM. Confocal imaging revealed that cortactin immunoreactivity is localized apposed to the presynaptic protein synaptophysin and Vglut, which suggests its presence in the postsynaptic compartment of excitatory synapses. Co-staining with a lipophilic dye that labels plasma membrane revealed the accumulation of cortactin in dendritic spines. Electron microscopy with immunoperoxidase labeling of cortactin revealed staining in dendrites which was denser within dendritic spines below PSDs, confirming the localization of cortactin within spines. In order to gain insight into the precise location of the protein, immunogold EM was performed. In agreement with previous results, immunogold labeling was prominent in spines, usually associated with electron-dense filaments, which suggests interaction with cytoskeleton. Analysis of the distribution of the immunogold particles within the spines revealed that these are localized 100-150nm away from the synapse, in the spine core. 11/8/2017 Pim - Based on prior knowledge of Cortactin and the statement "... usually associated with electron-dense filaments..." I allow annotation to the term 'postsynaptic actin cytoskeleton (GO:0098871)' without an IC construct. |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Confocal |
| Annotator(s): | Maria Andres-Alonso (ORCID:0000-0002-1585-539X) Michael Kreutz (ORCID:0000-0003-0575-6950) |
| Lab: | RG Neuroplasticity, Leibniz Institute for Neurobiology, 39118 Magdeburg, Germany and Leibniz Group 'Dendritic Organelles and Synaptic Function', Center for Molecular Neurobiology, ZMNH, University MC, Hamburg-Eppendorf, Hamburg, 20251, Germany |
| Additional literature: | Authors described Cortactin as a filamentous actin-binding protein. Literal: " (Cortactin) is present in multiple cell types and is enriched in the submembranous cortical region of adherent cells. The F-actin-binding region within cortactin maps to a domain comprised of five or six copies of a tan- demly repeated sequence. Deletion of a highly conserved carboxyl-terminal SH3 domain, a structural motif common to many cytoskeleton-associated proteins, did not affect the binding of cortactin to F-actin. The F-actin-binding activity, its cortical localization, and tyrosine phosphorylation suggest that cortactin may play a role in mediating microfilament- membrane interactions in response to signals propagated by receptor and/or nonreceptor protein tyrosine kinases." @ PMID:7680654 |
| SynGO annotation ID: | 995 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |