| Annotated protein: | Unconventional myosin-VI (Protein twist) (Unconventional myosin-6). Gene symbol: MYO6. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q64331 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ MYO6 |
| Ontology domain: | Cellular Component |
| SynGO term: | presynaptic endocytic zone (GO:0098833) |
| Synapse type(s): | cochlea Cochlear hair cell ribbon synapse |
| Annotated paper: | Roux I, et al. "Myosin VI is required for the proper maturation and function of inner hair cell ribbon synapses" Hum Mol Genet. 2009 Dec 1;18(23):4615-28 PMID:19744958 |
| Figure(s): | Fig1c-f |
| Annotation description: | Fig1 shows the localisation of Myo6 in the inner hair cell synapse. Quantification is given in the text (p4616). Light microscopy images show that Myo6 is present in throughout cellbody of the inner hair cell, with strong labelling at the basolateral face where the synapses are located. ImmunoEM shows that Myo6 is present at the cytoplasm (36%) and the synapse ribbon (16%), but most labelling is found at the edge of the active zone (48%; Fig1c-e) and on endocytic pits at the edge of the active zone (31% of active zone labelling; Fig1f). |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Confocal |
| Annotator(s): | Jan van Weering (ORCID:0000-0001-5259-4945) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | Fig4 shows that most of the endocytic pits (80nm) are located 33-60 nm from the edge of the active zone in mouse hippocampal synapses, indicative of the endocytic zone. @ PMID:24305055 |
| SynGO annotation ID: | 991 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |