| Annotated protein: | Actinin alpha 2. Gene symbol: ACTN2. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: D3ZCV0 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ ACTN2 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic actin cytoskeleton (GO:0098871) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Wyszynski M, et al. "Differential regional expression and ultrastructural localization of alpha-actinin-2, a putative NMDA receptor-anchoring protein, in rat brain" J Neurosci. 1998 Feb 15;18(4):1383-92 PMID:9454847 |
| Figure(s): | Fig. 2A, 5, 6 and 7 |
| Annotation description: | α-actinin-2 is found abundantly in crude synaptosomal membrane (Fig. 2A) α-Actinin-2 protein is localized to excitatory synapses (Fig. 5) Immunogold labeling for α-actinin-2 in postsynaptic densities (Fig. 6) Colocalization of α-actinin-2 with postsynaptic proteins by EM (Fig. 7). 28/6/2017 Pim - ACTN2 is localized in membrane and cytosolic synape preparations (Fig.2). - ACTN2 is localized to exc. synapses as shown by co-localization with Glur1 - ACTN2 is mainly located at PSD (membrane and cytoplasmic part) and on microfilaments (a.k.a actin) (Fig.6). - ACTN2 is colocalizing at the inner face of the PSD membrane with the NR1 subunit (Fig.7). |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Confocal Biochemical fractionation (generic) |
| Annotator(s): | Harold MacGillavry (ORCID:0000-0002-6153-3586) Casper Hoogenraad (ORCID:0000-0002-2666-0758) |
| Lab: | Department of Biology, Faculty of Science, Utrecht University, 3584 CH Utrecht, The Netherlands |
| Additional literature: | Knocked down a-actinin-2 in hippocampal neurons via short interfering (si)RNA shows results in immature, filopodia-like protrusions that fail to mature into a mushroom-shaped spine with a PSD during development. @ PMID:25007055 |
| SynGO annotation ID: | 988 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |