Annotated protein:Actinin alpha 2. Gene symbol: ACTN2. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: D3ZCV0
antibody wiki:
SynGO gene info:SynGO data @ ACTN2
Ontology domain:Cellular Component
SynGO term:postsynaptic actin cytoskeleton (GO:0098871)
Synapse type(s):hippocampus, glutamatergic
Annotated paper:Wyszynski M, et al. "Differential regional expression and ultrastructural localization of alpha-actinin-2, a putative NMDA receptor-anchoring protein, in rat brain" J Neurosci. 1998 Feb 15;18(4):1383-92 PMID:9454847
Figure(s):Fig. 2A, 5, 6 and 7
Annotation description:α-actinin-2 is found abundantly in crude synaptosomal membrane (Fig. 2A)
α-Actinin-2 protein is localized to excitatory synapses (Fig. 5)
Immunogold labeling for α-actinin-2 in postsynaptic densities (Fig. 6)
Colocalization of α-actinin-2 with postsynaptic proteins by EM (Fig. 7).

28/6/2017 Pim
- ACTN2 is localized in membrane and cytosolic synape preparations (Fig.2).
- ACTN2 is localized to exc. synapses as shown by co-localization with Glur1
- ACTN2 is mainly located at PSD (membrane and cytoplasmic part) and on microfilaments (a.k.a actin) (Fig.6).
- ACTN2 is colocalizing at the inner face of the PSD membrane with the NR1 subunit (Fig.7).
Evidence tracking, Biological System:Intact tissue
Cultured neurons
Evidence tracking, Protein Targeting:Antibody (detection)
Evidence tracking, Experiment Assay:Electron Microscopy
Confocal
Biochemical fractionation (generic)
Annotator(s):Harold MacGillavry (ORCID:0000-0002-6153-3586)
Casper Hoogenraad (ORCID:0000-0002-2666-0758)
Lab:Department of Biology, Faculty of Science, Utrecht University, 3584 CH Utrecht, The Netherlands
Additional literature:Knocked down a-actinin-2 in hippocampal neurons via short interfering (si)RNA shows results in immature, filopodia-like protrusions that fail to mature into a mushroom-shaped spine with a PSD during development. @ PMID:25007055
SynGO annotation ID:988
Dataset release (version):20231201
View annotation as GO-CAM model:Gene Ontology