| Annotated protein: | Dynein light chain 2, cytoplasmic (Dynein light chain LC8-type 2). Gene symbol: DYNLL2. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q78P75 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ DYNLL2 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic density (GO:0014069) |
| Synapse type(s): | hippocampus, glutamatergic cerebral cortex, glutamatergic |
| Annotated paper: | Naisbitt S, et al. "Interaction of the postsynaptic density-95/guanylate kinase domain-associated protein complex with a light chain of myosin-V and dynein" J Neurosci. 2000 Jun 15;20(12):4524-34 PMID:10844022 |
| Figure(s): | Fig. 5, 6 and 7 |
| Annotation description: | DLC antibody co-localizes with PSD-95 and F-actin at excitatory synapses in cultured hippocampal neurons (Fig. 5). Quantitative immunogold EM localization of DLC in the PSD and dendritic spine (Fig. 6A-H). Enrichment of DLC in PSD fractions (Fig. 6I). Colocalization of DLC with GKAP and PSD-95 by immunoEM (Fig. 7). The antibodies used in this study can not distinguish between DLC1 and DLC2 (98% similarity between these proteins) 3/11/2017 Pim - Based on the localization to the postsynapse of Venus-tagged Dlc2 the EM data with the antibody that does not distinguish between DLC1 and DLC2 I think this data on PSD localization can be transferred to the DLC2 protein. |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Microscopy (generic) Western blot Biochemical fractionation (generic) IP + WB/MSMS Y2H |
| Annotator(s): | Harold MacGillavry (ORCID:0000-0002-6153-3586) Casper Hoogenraad (ORCID:0000-0002-2666-0758) |
| Lab: | Department of Biology, Faculty of Science, Utrecht University, 3584 CH Utrecht, The Netherlands |
| Additional literature: | DLC2 was fused to the acceptor yellow fluorescent protein Venus (Venus-DLC2), was able to form dimers and colocalized in dendritic spines of cultured hippocampal neurons (Fig. 1A, Fig.S1B). @ PMID:22328512 |
| SynGO annotation ID: | 983 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |