| Annotated protein: | Calcium/calmodulin-dependent protein kinase type 1 (EC 2.7.11.17) (CaM kinase I) (CaM-KI) (CaM kinase I alpha) (CaMKI-alpha). Gene symbol: CAMK1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q63450 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CAMK1 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic density (GO:0014069) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Saneyoshi T, et al. "Activity-dependent synaptogenesis: regulation by a CaM-kinase kinase/CaM-kinase I/betaPIX signaling complex" Neuron. 2008 Jan 10;57(1):94-107 PMID:18184567 |
| Figure(s): | Figure S7 and Figure 4 |
| Annotation description: | The paper examines the signal transduction pathway where CaMKIalpha is involved. Since the end-result of the activation is a change in spine morphology, the authors examine the sub cellular localization of CaMKIalpha, to show that all components of the signaling pathway are located in the same sub cellular compartment. Figure S7 presents a WB analysis of an isolated PSD fraction and demonstrates a strong signal for CaMKIalpha using a specific antibody. **Figure 4b: Immunostaining showing the phosphorylated CaMKI is found in dendritic spines in cultured hippocampal neurons. However, the antibody is a 'pCaMKI' and there is no information regarding the isoform of CaMKI that it targets. 2/7/2017 Pim - As far as I can see there are not muliple isoforms of CamKI in Uniprot. Therefore, Fig.4b seems useful of annotation of CamKI as well. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal Western blot Biochemical fractionation (generic) |
| Annotator(s): | Noa Lipstein (ORCID:0000-0002-0755-5899) Cordelia Imig (ORCID:0000-0001-7351-8706) Vincent O'connor (ORCID:0000-0003-3185-5709) Nils Brose (ORCID:0000-0003-0938-8534) |
| Lab: | Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, 37075 Göttingen, Germany |
| SynGO annotation ID: | 979 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |