| Annotated protein: | Muscarinic acetylcholine receptor M1. Gene symbol: CHRM1. Taxonomy: Mus musculus (Mouse). Uniprot ID: P12657 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CHRM1 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of postsynaptic membrane (GO:0099055) |
| Synapse type(s): | cerebral cortex, acetylcholinergic hippocampus, acetylcholinergic |
| Annotated paper: | Yamasaki M, et al. "Preferential localization of muscarinic M1 receptor on dendritic shaft and spine of cortical pyramidal cells and its anatomical evidence for volume transmission" J Neurosci. 2010 Mar 24;30(12):4408-18 PMID:20335477 |
| Figure(s): | 1H, 3, 4 |
| Annotation description: | Data show quantitative immunogold labelling of postsynaptic structures in cortex (Figure 3) and hippocampus (Figure 4). Although clearly postsynaptic with accumulation at dendrites widespread receptor labelling is not juxtaposed to cholinergic release sites. This positioned to act as a postsynaptic sensor of volume transmission. Immunoreactivity controlled for specificity on knock out tissue. 17/8/2017 Pim - Based on the known transmembrane topology of the protein, the "integral component of ... membrane" term was chosen. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Noa Lipstein (ORCID:0000-0002-0755-5899) Cordelia Imig (ORCID:0000-0001-7351-8706) Vincent O'connor (ORCID:0000-0003-3185-5709) Nils Brose (ORCID:0000-0003-0938-8534) |
| Lab: | Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, 37075 Göttingen, Germany |
| SynGO annotation ID: | 978 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |