| Annotated protein: | Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A beta isoform (PP2A subunit A isoform PR65-beta) (PP2A subunit A isoform R1-beta). Gene symbol: PPP2R1B. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q7TNP2 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ PPP2R1B |
| Ontology domain: | Cellular Component |
| SynGO term: | synapse (GO:0045202) |
| Synapse type(s): | forebrain, glutamatergic |
| Annotated paper: | Biesemann C, et al. "Proteomic screening of glutamatergic mouse brain synaptosomes isolated by fluorescence activated sorting" EMBO J. 2014 Jan 13;33(2):157-70 PMID:24413018 |
| Figure(s): | Supplemental Table 2 |
| Annotation description: | The authors used synaptosomes derived from vGlut-Venus knockin mice to enrich for glutamatergic presynaptic terminals. Mass spectrometry was performed to detect protein content, which identified PPP2R1B. Although this method favors the detection of presynaptic proteins, postsynaptic or extrasynaptic localization of PPR2R1B cannot be excluded with this method. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | none of the above |
| Evidence tracking, Experiment Assay: | Mass-spectrometry Biochemical fractionation (generic) |
| Annotator(s): | Arthur de Jong (ORCID:0000-0002-7620-2704) Pascal Kaeser (ORCID:0000-0002-1558-1958) |
| Lab: | Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA |
| Additional literature: | Wilhelm et.al. deployed a similar technique based on a synaptosomal preparation and mass spectrometry. This study also detected PPP2R2A. @ PMID:24876496 |
| SynGO annotation ID: | 971 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |