| Annotated protein: | Abl interactor 1 (Abelson interactor 1) (Abi-1) (Eps8 SH3 domain-binding protein) (Eps8-binding protein) (e3B1). Gene symbol: ABI1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q9QZM5 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ ABI1 |
| Ontology domain: | Biological Process |
| SynGO term: | postsynapse to nucleus signaling pathway (GO:0099527) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Proepper C, et al. "Abelson interacting protein 1 (Abi-1) is essential for dendrite morphogenesis and synapse formation" EMBO J. 2007 Mar 7;26(5):1397-409 PMID:17304222 |
| Figure(s): | Fig 3, Fig 4, Fig 6a |
| Annotation description: | Abi-1 is found in the cytoplasm where it concentrates at PSDs and in the nucleus in NIH cells and also in hippocampal neurons. NMDA treatment induced an enhancement of nuclear Abi-1 staining which was accompanied by a decrease in the synaptic staining. This effect was also found in hippocampal neurons from acute rat brain slices. Moreover, WB analysis revealed an slight enrichment of Abi-1 in the nuclear fraction upon NMDA treatment. Nuclear translocation was prevented by treatment with colchicine or cytochalasinD, which suggests that the transport depends on microtubules and microfilaments. Nuclear entry seems to rely on Abi-1 phosphorylation of Y53 while synaptic localization seems to rely on the SH3 domain. Finally, Abi-1 influences transcriptional activity at the E-box regulated promoters in HeLa cells after EGF stimulation as part of a complex with Myc/Max. |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons Non-neuronal tissue |
| Evidence tracking, Protein Targeting: | Over-expression Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal Wide-field fluorescence Western blot Biochemical fractionation (generic) ELISA |
| Annotator(s): | Maria Andres-Alonso (ORCID:0000-0002-1585-539X) Michael Kreutz (ORCID:0000-0003-0575-6950) |
| Lab: | RG Neuroplasticity, Leibniz Institute for Neurobiology, 39118 Magdeburg, Germany and Leibniz Group 'Dendritic Organelles and Synaptic Function', Center for Molecular Neurobiology, ZMNH, University MC, Hamburg-Eppendorf, Hamburg, 20251, Germany |
| SynGO annotation ID: | 969 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |