| Annotated protein: | Contactin-5 (Neural recognition molecule NB-2). Gene symbol: CNTN5. Taxonomy: Mus musculus (Mouse). Uniprot ID: P68500 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CNTN5 |
| Ontology domain: | Cellular Component |
| SynGO term: | anchored component of presynaptic membrane (GO:0099026) |
| Synapse type(s): | cerebral cortex hippocampus |
| Annotated paper: | Shimoda Y, et al. "A cis-complex of NB-2/contactin-5 with amyloid precursor-like protein 1 is localized on the presynaptic membrane" Neurosci Lett. 2012 Feb 29;510(2):148-53 PMID:22285261 |
| Figure(s): | Figs. 3, 4 |
| Annotation description: | Fig 3: Contactin-5 / NB-2 in cultured hippocampal neurons co-localized with presynaptic synapsin. Fig 4: Synaptosomal fractions of P15 mouse cereral cortices analyzed by western blotting showed strong expression of contactin-5 / NB-2 in a fraction containing both presynaptic Kv1.2 and postsynaptic PSD95, and in a fraction containing only presynaptic Kv1.2 12/10/2017 Pim - Based on the known lipid anchor of the protein, the "anchored component of ... membrane" term was chosen. |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal Western blot Biochemical fractionation (generic) |
| Annotator(s): | Tony Cijsouw (ORCID:0000-0003-0912-1514) Thomas Biederer (ORCID:0000-0002-3670-7863) |
| Lab: | Department of Neuroscience, Tufts University School of Medicine, Boston, MA 02111, USA |
| Additional literature: | Contactin 5 expressed in HEK293 cells was significantly enriched at the cell membrane over the cytoplasm @ PMID:23519440 Fig 4: Wistar rats were purchased from Japan SLC (Shizuoka, Japan). Fig 4: In the rat brain auditory pathway, contactin-5 / NB-2 partially colocalizes with the presynaptic markers vGlut1 and vGlut2 in calyces of Held and neuropil. @ PMID:19177518 |
| SynGO annotation ID: | 958 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |