| Annotated protein: | 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (EC 3.1.4.11). Gene symbol: PLCB4. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q91UZ1 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ PLCB4 |
| Ontology domain: | Biological Process |
| SynGO term: | modulation of chemical synaptic transmission (GO:0050804) |
| Synapse type(s): | cerebellum, glutamatergic Parallel fibre (cerebellum -> Purkinje-cell) |
| Annotated paper: | Hirono M, et al. "Phospholipase Cbeta4 and protein kinase Calpha and/or protein kinase CbetaI are involved in the induction of long term depression in cerebellar Purkinje cells" J Biol Chem. 2001 Nov 30;276(48):45236-42 PMID:11551922 |
| Figure(s): | Figure 3, 4 |
| Annotation description: | The authors study the role of PLC b4 in synaptic transmission at cerebellar Purkinje cells. While basal synaptic transmission is normal (figure 3), long-term depression is abolished (Figure 4). Surprisingly, the phenotype was only observed in the rostral cerebellum (lobes 1-6), whereas in the caudal cerebellum (lobes 7-10), no obvious phenotype was observed. Caudal cerebellum may express other PLC isoforms that compensate for the loss of PLC b4 (Figure 2). PLC b4 expression in all cerebellar lobes, and successfull removal of PLC b4 expression, where shown by western blot (figure 2). |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Whole-cell patch clamp |
| Annotator(s): | Arthur de Jong (ORCID:0000-0002-7620-2704) Pascal Kaeser (ORCID:0000-0002-1558-1958) |
| Lab: | Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA |
| SynGO annotation ID: | 942 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |