| Annotated protein: | 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1 (EC 3.1.4.11) (PLC-154) (Phosphoinositide phospholipase C-beta-1) (Phospholipase C-beta-1) (PLC-beta-1). Gene symbol: PLCB1. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q9Z1B3 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ PLCB1 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of retrograde trans-synaptic signaling by endocanabinoid (GO:0099178) |
| Synapse type(s): | hippocampus, GABAergic |
| Annotated paper: | Hashimotodani Y, et al. "Phospholipase Cbeta serves as a coincidence detector through its Ca2+ dependency for triggering retrograde endocannabinoid signal" Neuron. 2005 Jan 20;45(2):257-68 PMID:15664177 |
| Figure(s): | Figure 2, 3 |
| Annotation description: | The authors investigate the role of PLC b1 in retrograde, endocannabinoid-mediated signalling in hippocampal neurons. They use paired recordings in culture, and stimulate with muscarinic receptor agonist oxo-M, which was previously shown to induce endocannabinoid-mediated inhibition of inhibitory transmission. In PLC-b1 knockout mice (PLCb1 -/-), oxo-M fails to suppres IPSC amplitude (Figure 2A). This is not due to a defect in presynaptic endocannabinoid receptors, since the synthetic cannabinoid 2-AG still induces IPSC suppression (Figure 2A). To demonstrate that this is a cell autonomous, postsynaptic effect, the authors superfuse the postsynaptic cell with various Ca2+ concentrations (PLCb1 requires Ca2+), and show that oxo-M does not suppress IPSCs at low intracellular calcium. Taken together, these data demonstrate that PLC b1 is required for transsynaptic, endocannabinoid-mediated signalling. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Electrophysiology (generic) |
| Annotator(s): | Arthur de Jong (ORCID:0000-0002-7620-2704) Pascal Kaeser (ORCID:0000-0002-1558-1958) |
| Lab: | Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA |
| SynGO annotation ID: | 940 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |