| Annotated protein: | Teneurin-2 (Ten-2) (Neurestin) (Protein Odd Oz/ten-m homolog 2) (Tenascin-M2) (Ten-m2) (Teneurin transmembrane protein 2) [Cleaved into: Ten-2, soluble form; Ten-2 intracellular domain (Ten-2 ICD)]. Gene symbol: TENM2. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q9R1K2-2 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ TENM2 |
| Ontology domain: | Cellular Component |
| SynGO term: | extrinsic component of postsynaptic membrane (GO:0098890) |
| Synapse type(s): | cerebral cortex, glutamatergic |
| Annotated paper: | Silva JP, et al. "Latrophilin 1 and its endogenous ligand Lasso/teneurin-2 form a high-affinity transsynaptic receptor pair with signaling capabilities" Proc Natl Acad Sci U S A. 2011 Jul 19;108(29):12113-8 PMID:21724987 |
| Figure(s): | Fig.3, Fig. 4 |
| Annotation description: | The authors use an antibody against the extracellular domain Lasso, a cleaved fragment of teneurin-2 that stays membrane-anchored, for immunoEM of synaptosomes. Fig.4 F,G,I shows the postsynaptic localization. This postsynaptic localization is supported by a biochemical sub-fractionation of synaptosomes shown in Fig.4H. Fig.3 shows the interaction of Lasso/LPH1 in NBa2 cells which supports a membrane localization of Lasso. 22/6/2017 Pim - "extrinsic component" --> Teneurin is cleaved after the TMR on the extracellular side. It is therefore not an integral protein. Yet, it stays membrane anchored as show in Figs. 3-5; this is probably through binding to cell surface proteins. - Accession Q9R1K2-2 was retrieved by BLAST of human lasso protein sequence against rat uniprot proteins. The best scoring reviewed sequence was selected with match score: E-value: 0.0 Score: 13616 Ident.: 97.8% Positives : 99.1% Query Length: 2637 Match Length: 2765 One unreviewed sequence (A0A096P6L6) had marginally better score but this seems to endode an equivalent lasso sequence. |
| Evidence tracking, Biological System: | Intact tissue Non-neuronal tissue |
| Evidence tracking, Protein Targeting: | Over-expression Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Western blot Biochemical fractionation (generic) Protein-protein interaction (generic) |
| Annotator(s): | Tony Cijsouw (ORCID:0000-0003-0912-1514) Thomas Biederer (ORCID:0000-0002-3670-7863) |
| Lab: | Department of Neuroscience, Tufts University School of Medicine, Boston, MA 02111, USA |
| Additional literature: | Lasso is constitutively cleaved within its ectodomain and the cleaved fragment remains partially anchored on the cell surface. @ PMID:27499734 |
| SynGO annotation ID: | 939 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |