| Annotated protein: | Contactin-2 (Axonal glycoprotein TAG-1) (Axonin-1) (Transient axonal glycoprotein 1) (TAX-1). Gene symbol: CNTN2. Taxonomy: Homo sapiens (Human). Uniprot ID: Q02246 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CNTN2 |
| Ontology domain: | Cellular Component |
| SynGO term: | anchored component of postsynaptic membrane (GO:0099025) |
| Synapse type(s): | hippocampus |
| Annotated paper: | Pinatel D, et al. "Inhibitory axons are targeted in hippocampal cell culture by anti-Caspr2 autoantibodies associated with limbic encephalitis" Front Cell Neurosci. 2015 Jul 9;9:265 PMID:26217189 |
| Figure(s): | Fig 8 |
| Annotation description: | Human contactin-2/TAG-1-GFP localized in cultured hippocampal neurons to areas apposed to presynaptic synaptophysin, consistent with a postsynaptic localization of contactin-2/TAG-1. In addition, a Caspr2-Fc fusion protein that can bind to its partner contactin-2/TAG-1 was applied to hippocampal neurons. The results indicated that contactin-2/TAG-1 is enriched in dendritic arbors and dendritic spines. 22/6/2017 Pim - membrane localization of TAG1 is inferred from the observation that TAG-1 mediates membrane association of Fc-Coupled Caspr2 and the known function of TAG-1 as a glycosylphosphatidylinositol-linked member of the Ig superfamily that acts as a cell adhesion molecule. - synapse term element is derived from co-localization with synaptophysin. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal Wide-field fluorescence |
| Annotator(s): | Tony Cijsouw (ORCID:0000-0003-0912-1514) Thomas Biederer (ORCID:0000-0002-3670-7863) |
| Lab: | Department of Neuroscience, Tufts University School of Medicine, Boston, MA 02111, USA |
| Additional literature: | A hydrophobic analysis of TAG-1 revealed a stretch of nonpolar amino acids at its extreme C-terminus, indicative of attachment to the membrane via a GPI lipid anchor. Membranes isolated from E15 brain were incubated in the presence or absence of phosphatidylinositol-specific phospholipase C (PI-PLC), an enzyme that releases lipid-anchored surface membrane proteins by cleavage of the GPI linkage. Released TAG-l was detected by immunoblotting after separation by SDS-PAGE. @ PMID:2317872 |
| SynGO annotation ID: | 937 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |