| Annotated protein: | PTPRF interacting protein alpha 2. Gene symbol: PPFIA2. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: F1M8A4 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ PPFIA2 |
| Ontology domain: | Biological Process |
| SynGO term: | structural constituent of presynapse (GO:0099181) |
| Synapse type(s): | hippocampus |
| Annotated paper: | Spangler SA, et al. "Liprin-alpha2 promotes the presynaptic recruitment and turnover of RIM1/CASK to facilitate synaptic transmission" J Cell Biol. 2013 Jun 10;201(6):915-28 PMID:23751498 |
| Figure(s): | Figure 5 |
| Annotation description: | An knockdown for liprin a2 (a2 KD) is using in rat hippocampal cultures to study its role in synapse morphology using electron microscopy. a2 KD induced a range of relatively mild effects on synapse morphology, including a reduction of the number of docked vesicles, active zone length and the density of vesicles. The total size of the vesicle cluster, however, was slightly increased. This suggests that liprin a2 plays a role in the assembly and/or maintenance of the presynapse, including the active zone. This is somewhat consistent with the phenotype of Liprin mutants in c.elegans (Zhen & Yin) and drosophila (Kaufman et.al.), although in these studies a broadening of the active zone was observed, with no effect on docking. The knockdown is validated in several ways. In western blot, the shRNA only affects 2a levels, without affecting other liprins (Figure 2 A-F). The shRNA also appears the liprin 2a signal at synapses in confocal microscopy (Figure 2D). Overexpression of liprin 2a can rescue the evoked excitatory current to near wild type levels (figure S1). An independent shRNA shows a similar phenotype (figure s1). |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | RNAi / shRNA |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Arthur de Jong (ORCID:0000-0002-7620-2704) Pascal Kaeser (ORCID:0000-0002-1558-1958) |
| Lab: | Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA |
| Additional literature: | Zhen & Yin observed a widening of the active zone in c. elegans liprin mutants. @ PMID:10517634 Kaufmann et.al. observed a widening of the active zone in drosophila liprin mutants. @ PMID:11931739 |
| SynGO annotation ID: | 936 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |