| Annotated protein: | PTPRF interacting protein alpha 2. Gene symbol: PPFIA2. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: F1M8A4 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ PPFIA2 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of synaptic vesicle exocytosis (GO:2000300) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Spangler SA, et al. "Liprin-alpha2 promotes the presynaptic recruitment and turnover of RIM1/CASK to facilitate synaptic transmission" J Cell Biol. 2013 Jun 10;201(6):915-28 PMID:23751498 |
| Figure(s): | Figure 2, 3, S1 |
| Annotation description: | An knockdown for liprin a2 (a2 KD) is using in rat hippocampal cultures to study its role in synaptic transmission. a2 KD induced a significant reduction in action-potential induced vesicle release as measured with electrophysiology (figure 3F). The response to hypertonic sucrose was also reduced (Figure 3G), suggesting in defect in priming. Besides the electrophysiology experiments, the authors also use two imaging methods (synaptophysin-Phluorin, Figure 3J-M) and FM dyes (Figure 2G-J) to demonstrate that 2a KD has a defect in vesicle fusion. The knockdown is validated in several ways. In western blot, the shRNA affects 2a levels without affecting other liprins (Figure 2 A-F). The shRNA also decreases the liprin 2a signal at synapses in confocal microscopy (Figure 2D). Overexpression of liprin 2a can rescue the excitatory evoked response to near wild type levels (figure S1). An independent shRNA shows a similar phenotype (figure s1). |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | RNAi / shRNA |
| Evidence tracking, Experiment Assay: | Wide-field fluorescence Whole-cell patch clamp |
| Annotator(s): | Arthur de Jong (ORCID:0000-0002-7620-2704) Pascal Kaeser (ORCID:0000-0002-1558-1958) |
| Lab: | Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA |
| SynGO annotation ID: | 935 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |