Annotated protein:Liprin-alpha 4 (Protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF),-interacting protein (liprin), alpha 4). Gene symbol: PPFIA4. Taxonomy: Mus musculus (Mouse). Uniprot ID: B8QI36
antibody wiki:
SynGO gene info:SynGO data @ PPFIA4
Ontology domain:Cellular Component
SynGO term:synapse (GO:0045202)
Synapse type(s):cerebral cortex
Annotated paper:Zurner M, et al. "Analyses of the spatiotemporal expression and subcellular localization of liprin-alpha proteins" J Comp Neurol. 2011 Oct 15;519(15):3019-39 PMID:21618221
Figure(s):Figure 2, 11, 12
Annotation description:The authors raised antibodies that recognize specific liprin alpha isoforms, and used these in confocal microscopy on cultured neurons. Specificity of the antibody is verified by western blot in HEK293 cells (Figure 2). Liprin a4 was found to colocalize to some extent with the presynaptic marker Bassoon (figure 11) and postsynaptic marker PSD-95 (Figure 12), suggesting a synaptic localization. There is a strong extrasynaptic, punctate component as well; and many Bassoon+ puncta do not contain Liprin a4. The synaptic liprin a4 levels are thus most likely low.

Spangler et.al. used a different antibody, and also found a synaptic localization of liprin a4 using confocal microscopy.
Evidence tracking, Biological System:Cultured neurons
Evidence tracking, Protein Targeting:Antibody (detection)
Evidence tracking, Experiment Assay:Confocal
Annotator(s):Arthur de Jong (ORCID:0000-0002-7620-2704)
Pascal Kaeser (ORCID:0000-0002-1558-1958)
Lab:Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA
Additional literature:Spangler et.al came to a similar conclusion using a different antibody. @ PMID:21618222
SynGO annotation ID:933
Dataset release (version):20231201
View annotation as GO-CAM model:Gene Ontology