Annotated protein:Liprin-alpha-3 (Protein tyrosine phosphatase receptor type f polypeptide-interacting protein alpha-3) (PTPRF-interacting protein alpha-3). Gene symbol: PPFIA3. Taxonomy: Mus musculus (Mouse). Uniprot ID: P60469
antibody wiki:
SynGO gene info:SynGO data @ PPFIA3
Ontology domain:Cellular Component
SynGO term:synapse (GO:0045202)
Synapse type(s):cerebral cortex
Annotated paper:Zurner M, et al. "Analyses of the spatiotemporal expression and subcellular localization of liprin-alpha proteins" J Comp Neurol. 2011 Oct 15;519(15):3019-39 PMID:21618221
Figure(s):Figure 2, 11, 12
Annotation description:The authors raised antibodies that recognize specific liprin alpha isoforms, and used these in confocal microscopy on cultured neurons. Specificity of the antibody is verified by western blot in HEK293 cells (Figure 2). Liprin a3 was found to colocalize with the presynaptic marker Bassoon (figure 11) and postsynaptic marker PSD-95 (Figure 12), suggesting a synaptic localization. There is, however, a clear extrasynaptic signal as well.

Spangler et.al. used a different antibody, and also found a synaptic localization of liprin a3 using confocal microscopy.
Evidence tracking, Biological System:Cultured neurons
Evidence tracking, Protein Targeting:Antibody (detection)
Evidence tracking, Experiment Assay:Confocal
Annotator(s):Arthur de Jong (ORCID:0000-0002-7620-2704)
Pascal Kaeser (ORCID:0000-0002-1558-1958)
Lab:Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA
Additional literature:Spangler et.al came to a similar conclusion using a different antibody. @ PMID:21618222
SynGO annotation ID:932
Dataset release (version):20231201
View annotation as GO-CAM model:Gene Ontology