| Annotated protein: | Liprin-alpha-2 (Protein tyrosine phosphatase receptor type f polypeptide-interacting protein alpha-2) (PTPRF-interacting protein alpha-2). Gene symbol: PPFIA2. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q8BSS9 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ PPFIA2 |
| Ontology domain: | Cellular Component |
| SynGO term: | synapse (GO:0045202) |
| Synapse type(s): | cerebral cortex |
| Annotated paper: | Zurner M, et al. "Analyses of the spatiotemporal expression and subcellular localization of liprin-alpha proteins" J Comp Neurol. 2011 Oct 15;519(15):3019-39 PMID:21618221 |
| Figure(s): | Figure 2, 11, 12 |
| Annotation description: | The authors raised antibodies that recognize specific liprin alpha isoforms, and used these in confocal microscopy on cultured neurons. Specificity of the antibody is verified by western blot in HEK293 cells (Figure 2). Liprin a2 was found to colocalize with the presynaptic marker Bassoon (figure 11) and postsynaptic marker PSD-95 (Figure 12), suggesting a synaptic localization. Two other studies are in line with a synaptic localization of liprin a2. Spangler et.al. used a different antibody, and also found a synaptic localization of liprin a2 using confocal microscopy. Wyszynski et.al. dectected liprin a2 at the pre- and postsynapse using immuno EM, although it is unclear whether this antibody has any crossreactivity with other liprin isoforms. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Arthur de Jong (ORCID:0000-0002-7620-2704) Pascal Kaeser (ORCID:0000-0002-1558-1958) |
| Lab: | Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA |
| Additional literature: | Spangler et.al came to a similar conclusion using a different antibody. @ PMID:21618222 Wyszynski detected liprin a2 in both the presynapse and postsynapse using immuno EM. @ PMID:11931740 |
| SynGO annotation ID: | 931 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |