| Annotated protein: | Epidermal growth factor receptor kinase substrate 8. Gene symbol: EPS8. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: F1M3L7 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ EPS8 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic density (GO:0014069) |
| Synapse type(s): | cerebellum, glutamatergic |
| Annotated paper: | Sekerkova G, et al. "Postsynaptic enrichment of Eps8 at dendritic shaft synapses of unipolar brush cells in rat cerebellum" Neuroscience. 2007 Mar 2;145(1):116-29 PMID:17223277 |
| Figure(s): | Figs. 6A, B, C |
| Annotation description: | Immunolocalization data of EM suggest that Eps8 is closely associated with the postsynaptic apparatus. Immunostaining of the PSDs was evident in both granule cell and unipolar brush cells (UBCs) dendrites in cerebellum. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Tony Cijsouw (ORCID:0000-0003-0912-1514) Thomas Biederer (ORCID:0000-0002-3670-7863) |
| Lab: | Department of Neuroscience, Tufts University School of Medicine, Boston, MA 02111, USA |
| Additional literature: | Key figures: Fig 1C; Fig 5B,C Eps8 was detected in biochemical fractionations, specifically in total cerebellar homogenate and the PSD fraction (synaptophysin-, PSD95+) as analyzed by immunoblotting. No specific mention is made of the species used for biochemical fractionations, but the remainder of the paper, including the Methods, only mentions use of mice. In cultured mouse cerebellar granule neuron, stained Eps8 colocalized with F-actin-rich clusters (stained by phalloidin) and NMDA receptor subunit NR2A-rich cluster. @ PMID:17018287 |
| SynGO annotation ID: | 929 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |