| Annotated protein: | Calcium/calmodulin-dependent protein kinase type IV (CaMK IV) (EC 2.7.11.17) (CaM kinase-GR) (Calspermin). Gene symbol: CAMK4. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P13234 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CAMK4 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynapse (GO:0098794) |
| Synapse type(s): | cerebral cortex, glutamatergic hippocampus, glutamatergic |
| Annotated paper: | Nakamura Y, et al. "An immunohistochemical study of Ca2+/calmodulin-dependent protein kinase IV in the rat central nervous system: light and electron microscopic observations" Neuroscience. 1995 Sep;68(1):181-94 PMID:7477923 |
| Figure(s): | Figure 15,16,17,18 |
| Annotation description: | The study utilizes a newly made antibody against Calcium /Calmodulin kinase IV and studies the protein's localization in neuronal tissue using pre-embedding DAB staining for electron microscopy. At the subcellular level, the authors report that Ca2+/calmodulin-dependent protein kinase IV-like immunoreactivity appeared in the nuclei of neurons in the various brain regions, and immunopositive reactivity, although less strong, is also observed in dendritic processes, but axon terminals do not show immunoreactivity. Figures 15-18 show profiles of axon terminals containing vesicles making synapses with immunoreactive dendrites. Figures 15, 16, 17 state that the staining is seen on microtubules. 30/10/2017 Pim - Because synapses are show close to the dendritic staining, I allow this annotation against term 'postsynapse (GO:0098794)'. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Noa Lipstein (ORCID:0000-0002-0755-5899) Cordelia Imig (ORCID:0000-0001-7351-8706) Vincent O'connor (ORCID:0000-0003-3185-5709) Nils Brose (ORCID:0000-0003-0938-8534) |
| Lab: | Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, 37075 Göttingen, Germany |
| SynGO annotation ID: | 924 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |