Annotated protein:Phospholipase C, beta 4. Gene symbol: PLCB4. Taxonomy: Mus musculus (Mouse). Uniprot ID: A2AT93
antibody wiki:
SynGO gene info:SynGO data @ PLCB4
Ontology domain:Cellular Component
SynGO term:postsynaptic SER
Synapse type(s):cerebellum
Annotated paper:Nakamura M, et al. "Signaling complex formation of phospholipase Cbeta4 with metabotropic glutamate receptor type 1alpha and 1,4,5-trisphosphate receptor at the perisynapse and endoplasmic reticulum in the mouse brain" Eur J Neurosci. 2004 Dec;20(11):2929-44 PMID:15579147
Figure(s):Figure 5, 6
Annotation description:The authors use an antibody specific for PLC B4 in immunoelectron microscopy studies. PLC B4 was detected at the postsynaptic ER in both pre-embedding EM(Figure 5). In line with a localization to the ER, in subcellular fractionation, PLC-B4 was detected in a fraction that is also enriched in IP3R (Figure 6).

The antibody against PLC B4 is verified by western blot against PLC B4 knockout (Figure 1).
Evidence tracking, Biological System:Intact tissue
Evidence tracking, Protein Targeting:Antibody (detection)
Evidence tracking, Experiment Assay:Electron Microscopy
Western blot
Biochemical fractionation (generic)
Annotator(s):Arthur de Jong (ORCID:0000-0002-7620-2704)
Pascal Kaeser (ORCID:0000-0002-1558-1958)
Lab:Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA
SynGO annotation ID:916
Dataset release (version):20231201
View annotation as GO-CAM model:Gene Ontology