Annotated protein: | Phospholipase C, beta 4. Gene symbol: PLCB4. Taxonomy: Mus musculus (Mouse). Uniprot ID: A2AT93 |
antibody wiki: | |
SynGO gene info: | SynGO data @ PLCB4 |
Ontology domain: | Cellular Component |
SynGO term: | postsynaptic SER |
Synapse type(s): | cerebellum |
Annotated paper: | Nakamura M, et al. "Signaling complex formation of phospholipase Cbeta4 with metabotropic glutamate receptor type 1alpha and 1,4,5-trisphosphate receptor at the perisynapse and endoplasmic reticulum in the mouse brain" Eur J Neurosci. 2004 Dec;20(11):2929-44 PMID:15579147 |
Figure(s): | Figure 5, 6 |
Annotation description: | The authors use an antibody specific for PLC B4 in immunoelectron microscopy studies. PLC B4 was detected at the postsynaptic ER in both pre-embedding EM(Figure 5). In line with a localization to the ER, in subcellular fractionation, PLC-B4 was detected in a fraction that is also enriched in IP3R (Figure 6). The antibody against PLC B4 is verified by western blot against PLC B4 knockout (Figure 1). |
Evidence tracking, Biological System: | Intact tissue |
Evidence tracking, Protein Targeting: | Antibody (detection) |
Evidence tracking, Experiment Assay: | Electron Microscopy Western blot Biochemical fractionation (generic) |
Annotator(s): | Arthur de Jong (ORCID:0000-0002-7620-2704) Pascal Kaeser (ORCID:0000-0002-1558-1958) |
Lab: | Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA |
SynGO annotation ID: | 916 |
Dataset release (version): | 20231201 |
View annotation as GO-CAM model: |