| Annotated protein: | Charged multivesicular body protein 2B. Gene symbol: CHMP2B. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: F1M8B7 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CHMP2B |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic density (GO:0014069) |
| Synapse type(s): | hippocampus |
| Annotated paper: | Chassefeyre R, et al. "Regulation of postsynaptic function by the dementia-related ESCRT-III subunit CHMP2B" J Neurosci. 2015 Feb 18;35(7):3155-73 PMID:25698751 |
| Figure(s): | 2b,c,d, 7b,c,d,e,f,g,h, Fig 8, Table 3 |
| Annotation description: | CHMP2B is found in dendritic shafts and spines where it colocalizes with PSD-95 as observed using confocal microscopy. Immunogold EM revealed significantly increased immunoreactivity in spines, where particles arranged beneath the spine membrane around the PSD. Subcellular fractionation revealed enrichment in membranes and synaptoneurosomes and PSD fractions, but could be solubilized from the PSD fraction by using Sarkosyl. Immunoprecipitation of CHMP2B from synaptoneurosomes also precipitates PSD scaffolds and this was also confirmed by mass spectrometry. |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Confocal Western blot Mass-spectrometry Biochemical fractionation (generic) IP + WB/MSMS |
| Annotator(s): | Maria Andres-Alonso (ORCID:0000-0002-1585-539X) Michael Kreutz (ORCID:0000-0003-0575-6950) |
| Lab: | RG Neuroplasticity, Leibniz Institute for Neurobiology, 39118 Magdeburg, Germany and Leibniz Group 'Dendritic Organelles and Synaptic Function', Center for Molecular Neurobiology, ZMNH, University MC, Hamburg-Eppendorf, Hamburg, 20251, Germany |
| Additional literature: | Literal: "We generated homozygous Chmp2b knockout mice (Chmp2b -/-) from a BayGenomics embryonic stem cell line with a genetrap insertion in intron 2 of Chmp2b (Fig. 1B). Analysis of protein levels showed that the knockout is incomplete (Fig. 1D), leading to an 84% reduction in CHMP2B levels (Fig. 1G). @ PMID:22366797 |
| SynGO annotation ID: | 892 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |