Annotated protein:Calcium/calmodulin-dependent protein kinase type II subunit beta (CaM kinase II subunit beta) (CaMK-II subunit beta) (EC 2.7.11.17). Gene symbol: CAMK2B. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P08413
antibody wiki:
SynGO gene info:SynGO data @ CAMK2B
Ontology domain:Cellular Component
SynGO term:postsynaptic density (GO:0014069)
Synapse type(s):forebrain
Annotated paper:Swulius MT, et al. "Structure and composition of the postsynaptic density during development" J Comp Neurol. 2010 Oct 15;518(20):4243-60 PMID:20878786
Figure(s):Figure 8, Table 2, Table 1
Annotation description:The paper characterizes the localization of Calcium /Calmodulin kinase II beta and alpha in isolated post-synaptic density fractions.

Figure 8 presents a quantification of the immunogold labeling as a function of age. Substantial labeling densities are present for both isoforms.

Table 2 presents the summary of the immunogold labeling quantifications.

Figure 1 presents the antibodies used. For all experiments, a negative control was made in the absence of primary antibody. The small amount of non-specific labeling was quantified and subtracted as background.

Figure S1 presents western blots for each of the antibodies. A single band is seen for the CaMKII alpha and beta, however, the blots are cropped so specificity evaluation is difficult.

19/8/2017 Pim
- The isolated also contain NMDAR subunits so in ference to the cytoplasmic part of the PSD is not valid.
Evidence tracking, Biological System:Intact tissue
Evidence tracking, Protein Targeting:Antibody (detection)
Evidence tracking, Experiment Assay:Electron Microscopy
Biochemical fractionation (generic)
Annotator(s):Noa Lipstein (ORCID:0000-0002-0755-5899)
Cordelia Imig (ORCID:0000-0001-7351-8706)
Vincent O'connor (ORCID:0000-0003-3185-5709)
Nils Brose (ORCID:0000-0003-0938-8534)
Lab:Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, 37075 Göttingen, Germany
SynGO annotation ID:888
Dataset release (version):20231201
View annotation as GO-CAM model:Gene Ontology