| Annotated protein: | Calbindin (Calbindin D28) (D-28K) (Spot 35 protein) (Vitamin D-dependent calcium-binding protein, avian-type). Gene symbol: CALB1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P07171 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CALB1 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic cytosol (GO:0099524) |
| Synapse type(s): | Calyx of Held |
| Annotated paper: | Felmy F, et al. "Developmental expression of the Ca2+-binding proteins calretinin and parvalbumin at the calyx of Held of rats and mice" Eur J Neurosci. 2004 Sep;20(6):1473-82 PMID:15355314 |
| Figure(s): | Figure 6 |
| Annotation description: | The paper is describing the expression pattern of Ca2+ binding proteins in the calyx of Held synapse using conventional immunostaining. The calyx of Held is a giant synapse where a clear separation of can be made between the pre and postsynaptic compartments using standard confocal microscopy. Figure 6 describes the results of a double immuostaining for Rab3 (a presynaptic marker) and calbindin. The staining for calbindin is restricted to the postsynaptic compartment, diffused (Fig. 6A), and does not co-localize with the Rab3 staining (Figure 6B,C). 1/7/2017 Pim Cytosolic localization is inferred from the diffuse staining of the protein. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Noa Lipstein (ORCID:0000-0002-0755-5899) Cordelia Imig (ORCID:0000-0001-7351-8706) Vincent O'connor (ORCID:0000-0003-3185-5709) Nils Brose (ORCID:0000-0003-0938-8534) |
| Lab: | Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, 37075 Göttingen, Germany |
| Additional literature: | The paper describes the synaptic connectivity of interneuronal subpopulations in the rat basolateral amygdalar nucleus. Figures 7, 12C and 13A,B show electron-microscopic images of immunoperoxidase reaction labeling calbindin at spines. @ PMID:12528187 |
| SynGO annotation ID: | 883 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |