| Annotated protein: | Cytohesin-2 (ARF nucleotide-binding site opener) (Protein ARNO) (PH, SEC7 and coiled-coil domain-containing protein 2) (CLM2) (SEC7 homolog B). Gene symbol: CYTH2. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P63035 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CYTH2 |
| Ontology domain: | Cellular Component |
| SynGO term: | extrinsic component of postsynaptic specialization membrane (GO:0098892) |
| Synapse type(s): | brain |
| Annotated paper: | Kitano J, et al. "Tamalin, a PDZ domain-containing protein, links a protein complex formation of group 1 metabotropic glutamate receptors and the guanine nucleotide exchange factor cytohesins" J Neurosci. 2002 Feb 15;22(4):1280-9 PMID:11850456 |
| Figure(s): | Fig. 6a |
| Annotation description: | In purified synaptosomes from rat brain, cytohesin-2 interacts with the PDZ domain-containing protein tamalin, which binds mGluR1 at the postsynaptic membrane. The binding of cytohesin-2 to tamalin was identified in a Y2H screen. Cytohesin-2 is enriched in PSD-fractions (Fig. 6a). PSD-95 and mGluR1 were mostly enriched in the PSDIII fraction, whereas tamalin and cytohesin-2 were enriched in the PSDII fraction, indicating a more loosely association with postsynaptic membranes. Antibody: Rabbit cytohesin-2 antisera were raised against an N-terminal sequence (aa 1-26). The antibody exhibited some (<5%) cross-reactivity with cytohesin-1 and -3. No KO-control |
| Evidence tracking, Biological System: | Intact tissue Non-neuronal tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Western blot Biochemical fractionation (generic) IP + WB/MSMS Y2H |
| Annotator(s): | Noa Lipstein (ORCID:0000-0002-0755-5899) Cordelia Imig (ORCID:0000-0001-7351-8706) Vincent O'connor (ORCID:0000-0003-3185-5709) Nils Brose (ORCID:0000-0003-0938-8534) |
| Lab: | Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, 37075 Göttingen, Germany |
| SynGO annotation ID: | 859 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |