| Annotated protein: | Sodium/calcium exchanger 3 (Na(+)/Ca(2+)-exchange protein 3) (Solute carrier family 8 member 3). Gene symbol: SLC8A3. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P70549 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ SLC8A3 |
| Ontology domain: | Biological Process |
| SynGO term: | calcium:cation antiporter activity involved in regulation of postsynaptic cytosolic calcium ion levels (GO:1905060) |
| Annotated paper: | Nicoll DA, et al. "Cloning of a third mammalian Na+-Ca2+ exchanger, NCX3" J Biol Chem. 1996 Oct 4;271(40):24914-21 PMID:8798769 |
| Figure(s): | Figure 6 |
| Annotation description: | The paper reports the cloning of the Na+/Ca2+ exchanger NCX3 and characterizes its transporter activity. Figure 6a reports an experiment where the NCX3 mRNA was injected in Xenopus oocytes (control- water injected). Na+ gradient-dependent labelled (45)Ca2+ uptake was measured under the following experimental conditions: -internal Na+ was elevated by incubation with nystatin. - the external solution contained labelled CaCl2 in a K+ medium, in the absence of external Na+. Figure 6b presents the results of a similar experiment but in BHK cells. The cells were transfected with the NCX3 DNA (control - non transfected cells). The specific Na+-dependent uptake of (45)Ca2+ is plotted. Under these conditions, the labeled Ca2+ uptake was larger than in the control. Background uptake levels were estimated by exchanging the external K+ ions for Na+, thereby eliminating the Na+ gradient which is necessary for Ca2+ uptake. Taken together, the experiment demonstrates that the identified cDNA codes for a Na+/Ca2+ exchanger. rule: NAS_MFCC-BP rationale: Protein has 'calcium:sodium antiporter activity (GO:0005432)' and is localized in the presynaptic membrane (show with high-resolution technique). The inference to term 'calcium:cation antiporter activity involved in regulation of postsynaptic cytosolic calcium ion levels (GO:1905060)' is trivial and non-controversial. Pinf_CC: [P70549] SynGO ID 843 (PMID:16914199, EM) |
| Evidence tracking, Biological System: | Non-neuronal tissue |
| Evidence tracking, Protein Targeting: | Over-expression |
| Evidence tracking, Experiment Assay: | Transmembrane transport assay |
| Non-tracable Author Statement (NAS): | This annotation required some inference over additional information/literature, and is not solely based on observations made in the annotated paper by its original authors. |
| Annotator(s): | Noa Lipstein (ORCID:0000-0002-0755-5899) Cordelia Imig (ORCID:0000-0001-7351-8706) Vincent O'connor (ORCID:0000-0003-3185-5709) Nils Brose (ORCID:0000-0003-0938-8534) |
| Lab: | Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, 37075 Göttingen, Germany |
| SynGO annotation ID: | 846 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |