| Annotated protein: | Sodium/calcium exchanger 2 (Na(+)/Ca(2+)-exchange protein 2) (Solute carrier family 8 member 2). Gene symbol: SLC8A2. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P48768 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ SLC8A2 |
| Ontology domain: | Biological Process |
| SynGO term: | calcium:cation antiporter activity involved in regulation of postsynaptic cytosolic calcium ion levels (GO:1905060) |
| Annotated paper: | Li Z, et al. "Cloning of the NCX2 isoform of the plasma membrane Na(+)-Ca2+ exchanger" J Biol Chem. 1994 Jul 1;269(26):17434-9 PMID:8021246 |
| Figure(s): | Figure 5a, b, d |
| Annotation description: | The paper reports the cloning and functional characterization of NCX2 as a Ca2+/Na+ exchanger. The authors use 'inside out' patches from Xenopus oocytes expressing NCX2. The patch pipette, facing the extracellular side, contains 8 mM Ca2+ and no Na+. The external solution, facing the cytoplasmic side of the membrane patch, contains 100 mM cesium. Figure 5a shows that a current can be elicited when the cesium is replaced by Na+, in the presence of 1, or 3 microMol Ca2+ in the external solution. When no Ca2+ is present at the external solution, no current is elicited, indicating a requirement of Ca2+ at the cytoplasmic domain of the exchanger. Figure 5b shows that when the Ca2+ is removed from the external solution, the current decays. Figure 5d shows the IV curve for the exchanger. The data presented indicates a function for NCX2 as a Na+/Ca2+ exchanger that is dependent on the ion gradients. rule: NAS_MFCC-BP rationale: Protein has 'calcium:sodium antiporter activity (GO:0005432)' and is localized in the presynaptic membrane (show with high-resolution technique). The inference to term 'calcium:cation antiporter activity involved in regulation of postsynaptic cytosolic calcium ion levels (GO:1905060)' is trivial and non-controversial. Pinf_CC: [P48768] SynGO ID 842 (PMID:16914199, EM) |
| Evidence tracking, Biological System: | Non-neuronal tissue |
| Evidence tracking, Protein Targeting: | Over-expression |
| Evidence tracking, Experiment Assay: | Electrophysiology (generic) |
| Non-tracable Author Statement (NAS): | This annotation required some inference over additional information/literature, and is not solely based on observations made in the annotated paper by its original authors. |
| Annotator(s): | Noa Lipstein (ORCID:0000-0002-0755-5899) Cordelia Imig (ORCID:0000-0001-7351-8706) Vincent O'connor (ORCID:0000-0003-3185-5709) Nils Brose (ORCID:0000-0003-0938-8534) |
| Lab: | Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, 37075 Göttingen, Germany |
| SynGO annotation ID: | 845 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |