Annotated protein:Receptor-type tyrosine-protein phosphatase S (R-PTP-S) (EC 3.1.3.48) (PTPNU-3) (Receptor-type tyrosine-protein phosphatase sigma) (R-PTP-sigma). Gene symbol: PTPRS. Taxonomy: Mus musculus (Mouse). Uniprot ID: B0V2N1
antibody wiki:
SynGO gene info:SynGO data @ PTPRS
Ontology domain:Cellular Component
SynGO term:integral component of presynaptic membrane (GO:0099056)
Synapse type(s):hippocampus, glutamatergic
Annotated paper:Takahashi H, et al. "Postsynaptic TrkC and presynaptic PTPsigma function as a bidirectional excitatory synaptic organizing complex" Neuron. 2011 Jan 27;69(2):287-303 PMID:21262467
Figure(s):Fig. 3e,f,j; Fig.4
Annotation description:Fig. 3e,f,j PTPdelta colocalizes with VGLUT1 but not with VGAT in rat hippocampal neurons. PTPdelta colocalize with PSD-95 in CA1stratumradiatum of mouse brain.
Literal
"PTPs immunoreactivity was also present in a punctate pattern decorating the dendrites of cultured hippocampal neurons at DIV 15 and these puncta overlapped with VGLUT1 (Figure 3E). PTPs puncta overlapping VGLUT1 were also observed on axons not contacting dendrites, suggesting an axonal localization (Figure 3E, arrowheads). PTPs puncta were not colocalized with VGAT clusters (Figure 3F). Furthermore, PTPs puncta were apposed to PSD-95 puncta in brain, as shown here for hippocampal CA1 region (Figure 3J). Thus, endogenous PTPs is also localized to excitatory synaptic sites in vitro and in vivo."

Fig.4 because PTPdelta interect with TrkC, the figure shows that TrkC and PTPs ectodomains, linked to beats, induce excitatory presynaptic and postsynaptic differentiation, respectively.
Literal
To test whether PTPs mediates TrkC induced presynaptic differentiation, we investigated synapsin clustering around
TrkC-Fc-coated beads that contacted axons expressing either HA-PTPs or HA-PTPs lacking the intracellular domain
(HA-PTPsDICD). TrkC-Fc-coated beads induced HA-PTPs clustering accompanied by synapsin clustering on axons (Figures 4D and 4E). TrkC-induced synapsin clustering associated with HAPTPs was equivalent to TrkC-induced synapsin clustering on
neighboring nontransfected axons (Figure 4E), suggesting that
HA-PTPs is comparable in activity to the endogenous presynaptic
receptor of TrkC. In contrast, TrkC-Fc-coated beads that
induced HA-PTPsDICD clustering on axons did not induce simultaneous clustering of synapsin (Figures 4D and 4E). Presumably, HA-PTPsDICD effectively competed with endogenous PTPs for TrkC binding and blocked transmembrane signaling from TrkC to axonal intracellular targets for presynaptic differentiation. Taken together, these data suggest that PTPs is an axonal receptor for TrkC that triggers presynaptic differentiation.
Next, we tested whether PTPs ectodomain triggers excitatory
postsynaptic differentiation associated with dendritic accumulation of TrkC. PTPs-Fc-coated beads that contacted dendrites induced clustering of endogenous dendritic TrkC with NMDA receptor subunit NR1 (Figure 4F). NR1 clusters induced by
PTPs-Fc-coated beads were not apposed to synapsin (Figure
4G), indicating that these NR1 clusters were not associated with interneuronal synapses. PTPs-Fc-coated beads also induced clustering of PSD-95 but not of gephyrin (Figures 4H and 4I). We also confirmed in the coculture assay that COS cells expressing PTPs-CFP induced clustering of NR1 (data not shown) and of PSD-95 but not of gephyrin (Figure S4G) on contacting
dendrites. These data indicate that PTPs acts as a presynaptic factor to induce excitatory postsynaptic differentiation
and suggest that its postsynaptic receptor is TrkC."

7/11/2017 Pim
- Based on the known transmembrane topology of the protein, the "integral component of ... membrane" term was chosen.
Evidence tracking, Biological System:Intact tissue
Cultured neurons
Evidence tracking, Protein Targeting:Antibody (detection)
Evidence tracking, Experiment Assay:Confocal
Annotator(s):Chiara Verpelli (ORCID:0000-0003-2949-9725)
Carlo Sala (ORCID:0000-0003-0662-9523)
Lab:CNR Neuroscience Institute Milan and Dept. of Biotechnology and Translational Medicine, University of Milan, 20129 Milan, Italy
SynGO annotation ID:844
Dataset release (version):20231201
View annotation as GO-CAM model:Gene Ontology