| Annotated protein: | Sodium/calcium exchanger 3 (Na(+)/Ca(2+)-exchange protein 3) (Solute carrier family 8 member 3). Gene symbol: SLC8A3. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P70549 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ SLC8A3 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of postsynaptic membrane (GO:0099055) |
| Synapse type(s): | hippocampus cerebral cortex |
| Annotated paper: | Minelli A, et al. "Cellular and subcellular localization of Na+-Ca2+ exchanger protein isoforms, NCX1, NCX2, and NCX3 in cerebral cortex and hippocampus of adult rat" Cell Calcium. 2007 Mar;41(3):221-34 PMID:16914199 |
| Figure(s): | Figure 6 and Table 1 |
| Annotation description: | Ultrastructural localization of NCX3 in the cerebral cortex and hippocampus. Preembedding immunoperoxidase and silver-enhanced immunogold procedures were used. Figure 6b: in CA3 stratum radiatum, several dendritic profiles contain clumps of immunoreactivity associated to microtubules, mitochondria and to plasmalemma. 6c: immunogold labelling associated with the plasma membrane near the postsynaptic density in a dendrite. 6d,e: labelled dendritic spines. Table 1 presents a quantification of the immunolabeling, and shows that the majority of NCX3 profiles the cerebral cortex and in hippocampus are in dendrites and spines. Antibody: 'a rabbit polyclonal IgG antibody generously provided by Dr. K.D. Philipson'. Figure 1 demonstrated antibody specificity in western blotting of hippocampus, heart and cortex homogenates in comparison with a recombinant protein produced in HEK cells. Strong WB signals with little cross-reactivity are a good indication for the antibody specificity. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Noa Lipstein (ORCID:0000-0002-0755-5899) Cordelia Imig (ORCID:0000-0001-7351-8706) Vincent O'connor (ORCID:0000-0003-3185-5709) Nils Brose (ORCID:0000-0003-0938-8534) |
| Lab: | Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, 37075 Göttingen, Germany |
| SynGO annotation ID: | 843 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |