| Annotated protein: | Sodium/calcium exchanger 1 (Na(+)/Ca(2+)-exchange protein 1) (Solute carrier family 8 member 1). Gene symbol: SLC8A1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q01728 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ SLC8A1 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of postsynaptic membrane (GO:0099055) |
| Synapse type(s): | hippocampus cerebral cortex |
| Annotated paper: | Minelli A, et al. "Cellular and subcellular localization of Na+-Ca2+ exchanger protein isoforms, NCX1, NCX2, and NCX3 in cerebral cortex and hippocampus of adult rat" Cell Calcium. 2007 Mar;41(3):221-34 PMID:16914199 |
| Figure(s): | Figure 4 and table 1 |
| Annotation description: | Ultrastructural localization of NCX1 in the cerebral cortex and hippocampus CA1. Preembedding immunoperoxidase and silver-enhanced immunogold procedures were used. Figure 4b shows NCX1 immunoparticles associated to microtubules, mitochondria, and plasma membrane in several dendritic profiles. Figure 4C shows dendritic spines labelled by the DAB reaction, indicating expression of NCX1. The corresponding axons are not labelled. Figure 4D - particles associated with the membrane in the vicinity of the PSD in the CA1 stratum radiatum. Table 1 presents a quantification of the immunolabeling, and shows that a large portion of NCX1 profiles the cerebral cortex and in the hippocampus are in dendrites and spines. Antibody:'NCX1 protein was detected by using a commercially available mouse monoclonal IgG antibody (R3F1; Swant, Bellinzona, Switzerland)'. Figure 1 demonstrated antibody specificity in western blotting of hippocampus, heart and cortex homogenates in comparison with a recombinant protein produced in HEK cells. Strong WB signals with little cross-reactivity are a good indication for the antibody specificity. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Noa Lipstein (ORCID:0000-0002-0755-5899) Cordelia Imig (ORCID:0000-0001-7351-8706) Vincent O'connor (ORCID:0000-0003-3185-5709) Nils Brose (ORCID:0000-0003-0938-8534) |
| Lab: | Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, 37075 Göttingen, Germany |
| SynGO annotation ID: | 841 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |