| Annotated protein: | Sodium/calcium exchanger 1 (Na(+)/Ca(2+)-exchange protein 1) (Solute carrier family 8 member 1). Gene symbol: SLC8A1. Taxonomy: Canis lupus familiaris (Dog). Uniprot ID: P23685 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ SLC8A1 |
| Ontology domain: | Biological Process |
| SynGO term: | calcium:cation antiporter activity involved in regulation of postsynaptic cytosolic calcium ion levels (GO:1905060) |
| Annotated paper: | Nicoll DA, et al. "Molecular cloning and functional expression of the cardiac sarcolemmal Na(+)-Ca2+ exchanger" Science. 1990 Oct 26;250(4980):562-5 PMID:1700476 |
| Figure(s): | Figure 1 |
| Annotation description: | The paper reports the cloning of the Na+/Ca2+ exchanger (NCX1) from canine and characterizes its transporter activity. Figure 1 reports an experiment where the NCX1 mRNA was injected in Xenopus oocytes (control- water injected). Na+ gradient-dependent labelled (45)Ca2+ uptake was measured under the following experimental conditions: -internal Na+ was elevated by incubation with nystatin. - the external solution contained 10 uM of labelled CaCl2 in a K+ medium, in the absence of external Na+. Under these conditions, the labeled Ca2+ uptake was at least one order of magnitude greater than control or than previously observed in oocytes injected with poly(A)+ RNA from cardiac tissue (PMID: 2462361). When the external K+ ions were exchanged for Na+, or when the nystatin treatment was omitted, only background uptake levels were observed, indicating the need for a Na+ gradient for the Ca2+ uptake. Taken together, the experiment demonstrates that the identified cDNA codes for a Na+/Ca2+ exchanger. rule: NAS_MFCC-BP rationale: Protein has 'calcium:sodium antiporter activity (GO:0005432)' and is localized in the presynaptic membrane (show with high-resolution technique). The inference to term 'calcium:cation antiporter activity involved in regulation of postsynaptic cytosolic calcium ion levels (GO:1905060)' is trivial and non-controversial. Pinf_CC: [Q01728] SynGO ID 841 (PMID:16914199, EM) |
| Evidence tracking, Biological System: | Non-neuronal tissue |
| Evidence tracking, Protein Targeting: | Over-expression |
| Evidence tracking, Experiment Assay: | Transmembrane transport assay |
| Non-tracable Author Statement (NAS): | This annotation required some inference over additional information/literature, and is not solely based on observations made in the annotated paper by its original authors. |
| Annotator(s): | Noa Lipstein (ORCID:0000-0002-0755-5899) Cordelia Imig (ORCID:0000-0001-7351-8706) Vincent O'connor (ORCID:0000-0003-3185-5709) Nils Brose (ORCID:0000-0003-0938-8534) |
| Lab: | Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, 37075 Göttingen, Germany |
| SynGO annotation ID: | 839 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |