| Annotated protein: | Sodium-dependent dopamine transporter (DA transporter) (DAT) (Solute carrier family 6 member 3). Gene symbol: SLC6A3. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P23977 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ SLC6A3 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of postsynaptic membrane (GO:0099055) |
| Synapse type(s): | midbrain, dopaminergic |
| Annotated paper: | Nirenberg MJ, et al. "The dopamine transporter is localized to dendritic and axonal plasma membranes of nigrostriatal dopaminergic neurons" J Neurosci. 1996 Jan 15;16(2):436-47 PMID:8551328 |
| Figure(s): | Fig. 1-3 |
| Annotation description: | The dopamine transporter is an integral membrane protein of the postsynaptic membrane. Pre-embedding immunogold and immunoperoxidase labelling demonstrates DAT-immunoreactivity predominantly along the plasma membrane in dendrites and dendritic spines (Fig. 1-3). The majority of the DAT-immunoreactivity was localized to tyrosine-hydroxylase positive neurons. The DAT antiserum is a polyclonal antiserum directed against a synthetic peptide (aa42-59; LTNSTLINPPQTPVEAQE). The specificity of the antibody was demonstrated in a previous study (See reference 1). No KO-control. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Noa Lipstein (ORCID:0000-0002-0755-5899) Cordelia Imig (ORCID:0000-0001-7351-8706) Vincent O'connor (ORCID:0000-0003-3185-5709) Nils Brose (ORCID:0000-0003-0938-8534) |
| Lab: | Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, 37075 Göttingen, Germany |
| Additional literature: | Generation and characterization of the antibody used. @ PMID:7499533 |
| SynGO annotation ID: | 835 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |