| Annotated protein: | Myristoylated alanine-rich C-kinase substrate (MARCKS) (Protein kinase C substrate 80 kDa protein). Gene symbol: MARCKS. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P30009 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ MARCKS |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of modification of postsynaptic actin cytoskeleton (GO:1905274) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Calabrese B, et al. "Essential role for the PKC target MARCKS in maintaining dendritic spine morphology" Neuron. 2005 Oct 6;48(1):77-90 PMID:16202710 |
| Figure(s): | Fig.1, 2, 3 |
| Annotation description: | Rat hippocampal neurons expressing MARCKS RNAi constructs exhibit a greatly reduced spine density, spine length, and spine head width (Fig.1B). Efficiency of the KD was assessed by ICC (Fig. 1A). MARCKS influences number of spines and spine morphology in a phosphorylation-dependent manner. Overexpression of MARCKS constructs that mimic constitutively phosphorylated MARCKS (S4D-MARCKS and Dbl-MARCKS) and therefore have reduced membrane-association than wildtype-MARCKS in neurons causes a reduced spine protrusion length and head width (Fig.2). Overexpression of a MARCKS construct that mimic constitutively phosphorylated MARCKS (S4D-MARCKS) causes a reduction in total F-actin in dendrites in comparison to control neurons (Fig. 3A,B). |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | RNAi / shRNA Over-expression |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Noa Lipstein (ORCID:0000-0002-0755-5899) Cordelia Imig (ORCID:0000-0001-7351-8706) Vincent O'connor (ORCID:0000-0003-3185-5709) Nils Brose (ORCID:0000-0003-0938-8534) |
| Lab: | Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, 37075 Göttingen, Germany |
| SynGO annotation ID: | 829 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |