Annotated protein:	Myristoylated alanine-rich C-kinase substrate (MARCKS) (Protein kinase C substrate 80 kDa protein). Gene symbol: MARCKS. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P30009
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SynGO gene info:	SynGO data @ MARCKS
Ontology domain:	Cellular Component
SynGO term:	anchored component of postsynaptic membrane (GO:0099025)
Synapse type(s):	cerebral cortex hypothalamus
Annotated paper:	Ouimet CC, et al. "Localization of the MARCKS (87 kDa) protein, a major specific substrate for protein kinase C, in rat brain" J Neurosci. 1990 May;10(5):1683-98 PMID:2332803
Figure(s):	Fig.11-13
Annotation description:	Preembedding immuno-EM reveals the presence of immunoreactivityfor MARCKS in some dendrites and spines in the cingulate cortex and hypothalamus (Fig.11-13). Heavy immunoreactivity was often associated with the cytoplasmic surface of the plasma membrane (Fig 11A, Fig. 13A). MARCKS is myristoylated and interacts additionally via a cluster of basic residues through electrostatic interactions with the plasma membrane (myristoyl-electrostatic switch). Therefore the term "anchored component of the postsynaptic PM" was chosen. An affinity purified antibody against rat MARCKS was generated in the study and the specificity of the antibody was confirmed by Western Blot (Fig. 1). No KO-control. 21/8/2017 Pim - myristoylation used to infer 'anchored component of presynaptic membrane (GO:0099026)'
Evidence tracking, Biological System:	Intact tissue
Evidence tracking, Protein Targeting:	Antagonist / agonist
Evidence tracking, Experiment Assay:	Electron Microscopy
Annotator(s):	Noa Lipstein (ORCID:0000-0002-0755-5899) Cordelia Imig (ORCID:0000-0001-7351-8706) Vincent O'connor (ORCID:0000-0003-3185-5709) Nils Brose (ORCID:0000-0003-0938-8534)
Lab:	Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, 37075 Göttingen, Germany
SynGO annotation ID:	828
Dataset release (version):	20231201
View annotation as GO-CAM model: