| Annotated protein: | Myristoylated alanine-rich C-kinase substrate (MARCKS) (Protein kinase C substrate 80 kDa protein). Gene symbol: MARCKS. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P30009 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ MARCKS |
| Ontology domain: | Cellular Component |
| SynGO term: | presynaptic cytosol (GO:0099523) |
| Synapse type(s): | cerebral cortex |
| Annotated paper: | Ouimet CC, et al. "Localization of the MARCKS (87 kDa) protein, a major specific substrate for protein kinase C, in rat brain" J Neurosci. 1990 May;10(5):1683-98 PMID:2332803 |
| Figure(s): | Fig.11 |
| Annotation description: | Preembedding immuno-EM reveals the presence of immunoreactivityfor MARCKS in some axons and axon terminals in the cingulate cortex. MARCKS is myristoylated and interacts additionally via a cluster of basic residues through electrostatic interactions with the plasma membrane (myristoyl-electrostatic switch). Phosphorylation of MARCKS induces translocation of the protein to the cytosol (Reference 1). MARCKS is detected both, in membrane and soluble fractions (Reference 2). An affinity purified antibody against rat MARCKS was generated in the study and the specificity of the antibody was confirmed by Western Blot (Fig. 1). No KO-control. 21/8/2017 Pim - Literal (abstract): "Electron microscopic analysis revealed immunoreactivity in axons, axon terminals, small dendritic branches, and occasionally in dendritic spines. In neuronal processes, immunoreactivity was particularly prominent in association with microtubules, but reaction product was also seen in cytosol and adjacent to plasma membranes. No reaction product was observed in large dendrites, somata, or nuclei." |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antagonist / agonist |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Noa Lipstein (ORCID:0000-0002-0755-5899) Cordelia Imig (ORCID:0000-0001-7351-8706) Vincent O'connor (ORCID:0000-0003-3185-5709) Nils Brose (ORCID:0000-0003-0938-8534) |
| Lab: | Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, 37075 Göttingen, Germany |
| Additional literature: | In isolated synaptosomes, phosphorylation of MARCKS by PKC in response to stimulation by phorbol esters or depolarization-induced Ca2+-influx, induces a translocation of MARCKS from the membrane to the cytosol (Fig.1-3). @ PMID:2928330 In subcellular fractionation experiments of rat brain, MARCKS was enriched in soluble fractions (S3 and LS2), but also present in most membrane fractions, however was not detected in the SV fraction (Fig.5). @ PMID:8557647 |
| SynGO annotation ID: | 826 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |