| Annotated protein: | Leucine-rich glioma-inactivated protein 1. Gene symbol: LGI1. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q9JIA1 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ LGI1 |
| Ontology domain: | Cellular Component |
| SynGO term: | synaptic cleft (GO:0043083) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Fukata Y, et al. "Disruption of LGI1-linked synaptic complex causes abnormal synaptic transmission and epilepsy" Proc Natl Acad Sci U S A. 2010 Feb 23;107(8):3799-804 PMID:20133599 |
| Figure(s): | Fig. 2c, Fig. 5e,f, Fig. S3a |
| Annotation description: | Fig. 2c, The Thy1 promoter-driven LGI1-FH protein was functionally secreted and bound to neurons that express ADAM22. LGI1 is a secreted proteins that binds to the postsynaptic protein ADAM22 Literal "the LGI1 point mutation (E383A) observed in patients with ADPEAF prevented its neuronal secretion and binding to ADAM22 in hippocampal neurons, whereas secreted wild-type LGI1 bound to ADAM22 at dendritic spines (Fig. 2C)." Fig. 5e,f, Immunofluorescence labeling of LGI1 shows that LGI1 with PSD-95 at the molecular layer of the hippocampal regions. Immuno-EM of hippocampal dentate gyrus shows that LGI1 localizes at the synaptic site. Literal "On immunofluorescent microscopic examination, LGI1 was detected as tiny puncta at the molecular layers of the hippocampal regions (Fig. 5E). LGI1 was often apposed or colocalized with PSD-95. Furthermore, immunoelectron microscopic analysis showed that gold particles for LGI1 were often detected at or around the synaptic cleft in the hippocampal dentate gyrus region (Fig. 5F, arrowheads)" Fig. S3a, LGI1, is enriched at both both in Triton X-100-soluble presynaptic and insoluble PSD fractions Literal "We next asked whether LGI1 controls subcellular distributions of ADAM22, ADAM23, and Kv1. LGI1, ADAM22, and ADAM23 similarly distributed both presynaptically and postsynaptically (Fig. S3A)." |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Confocal Biochemical fractionation (generic) |
| Annotator(s): | Chiara Verpelli (ORCID:0000-0003-2949-9725) Carlo Sala (ORCID:0000-0003-0662-9523) |
| Lab: | CNR Neuroscience Institute Milan and Dept. of Biotechnology and Translational Medicine, University of Milan, 20129 Milan, Italy |
| SynGO annotation ID: | 823 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |