Annotated protein:	Sodium-dependent serotonin transporter (SERT) (5HT transporter) (5HTT) (Solute carrier family 6 member 4). Gene symbol: SLC6A4. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q60857
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SynGO gene info:	SynGO data @ SLC6A4
Ontology domain:	Biological Process
SynGO term:	neurotransmitter reuptake (GO:0098810)
Annotated paper:	Chang AS, et al. "Cloning and expression of the mouse serotonin transporter" Brain Res Mol Brain Res. 1996 Dec 31;43(1-2):185-92 PMID:9037532
Figure(s):	Fig.2
Annotation description:	The 5-HT transporter (SERT) is an integral membrane protein that transports 5-HT into cells in a sodium and chloride-dependent manner. COS-1 cells were transfected to transiently express mouse SERT. Transport of radiolabelled [H3]5-HT into COS-1 cells (Fig.2a) is dependent on the extracellular sodium (Fig.3b) and chloride (Fig.2c) concentration. rule: NAS_MFCC-BP rationale: Protein has "serotonin transmembrane transporter activity" and is localized in the presynaptic membrane (show with high-resolution technique). The inference to term 'neurotransmitter uptake (GO:0001504)' is trivial and non-controversial. Pinf_CC: [P31652] SynGO ID 820 (PMID:10460242, EM)
Evidence tracking, Biological System:	Non-neuronal tissue
Evidence tracking, Protein Targeting:	Over-expression
Evidence tracking, Experiment Assay:	Transmembrane transport assay
Non-tracable Author Statement (NAS):	This annotation required some inference over additional information/literature, and is not solely based on observations made in the annotated paper by its original authors.
Annotator(s):	Noa Lipstein (ORCID:0000-0002-0755-5899) Cordelia Imig (ORCID:0000-0001-7351-8706) Vincent O'connor (ORCID:0000-0003-3185-5709) Nils Brose (ORCID:0000-0003-0938-8534)
Lab:	Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, 37075 Göttingen, Germany
SynGO annotation ID:	819
Dataset release (version):	20231201
View annotation as GO-CAM model: