| Annotated protein: | Actin, cytoplasmic 2 (EC 3.6.4.-) (Gamma-actin) [Cleaved into: Actin, cytoplasmic 2, N-terminally processed]. Gene symbol: ACTG1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P63259 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ ACTG1 |
| Ontology domain: | Biological Process |
| SynGO term: | structural constituent of postsynaptic actin cytoskeleton (GO:0098973) |
| Synapse type(s): | cerebellum |
| Annotated paper: | Micheva KD, et al. "beta-Actin is confined to structures having high capacity of remodelling in developing and adult rat cerebellum" Eur J Neurosci. 1998 Dec;10(12):3785-98 PMID:9875357 |
| Figure(s): | Fig 4B, 5A, 6A, 7A, 8B, 9A and Table 1 |
| Annotation description: | Electron microscopy of post-embedding immunogold-labelled Rat cerebellar tissue sections (from P5, P12 and adult) stained with an antibody specific for γ-actin. γ-actin was localized to both the presynapse and postsynapse, but was especially present in the dendritic spines. 9/6/2017 Pim Ticket made under IC flag since inferred form known function of actin and the actin cytoskeleton. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Ryan J. Farrell (ORCID:0000-0003-4022-8707) Ghazaleh Ashrafi (ORCID:0000-0001-7480-0826) Camila Pulido (ORCID:0000-0002-5648-066X) Jaime de Juan-Sanz (ORCID:0000-0002-1212-5623) Timothy Ryan (ORCID:0000-0003-2533-9548) |
| Lab: | Department of Biochemistry, Weill Cornell Medicine, New York, NY 10065, USA |
| Additional literature: | This paper verifies that the gamma-actin antibody used in the above reference only recognizes the gamma-actin form and not the ?-actin or ?-actin form. See especially Figure 4. @ PMID:2422178 |
| SynGO annotation ID: | 816 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |