Annotated protein:	Actin, cytoplasmic 1 (EC 3.6.4.-) (Beta-actin) [Cleaved into: Actin, cytoplasmic 1, N-terminally processed]. Gene symbol: ACTB. Taxonomy: Homo sapiens (Human). Uniprot ID: P60709
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SynGO gene info:	SynGO data @ ACTB
Ontology domain:	Cellular Component
SynGO term:	postsynaptic actin cytoskeleton (GO:0098871)
Synapse type(s):	hippocampus, glutamatergic
Annotated paper:	Honkura N, et al. "The subspine organization of actin fibers regulates the structure and plasticity of dendritic spines" Neuron. 2008 Mar 13;57(5):719-29 PMID:18341992
Figure(s):	Fig 1,2
Annotation description:	The authors use a fluorescently labeled overexpression of human b-actin to study the dynamics (G-actin/F-actin transitions) of the actin cytoskeleton in dendritic spines. As inferred by the curator and based on the author's intent it is derived that the b-actin gene provides the structural basis of the F-actin skeleton at the postsynapse.
Evidence tracking, Biological System:	Cultured neurons
Evidence tracking, Protein Targeting:	Over-expression
Evidence tracking, Experiment Assay:	Confocal
Annotator(s):	Ryan J. Farrell (ORCID:0000-0003-4022-8707) Ghazaleh Ashrafi (ORCID:0000-0001-7480-0826) Camila Pulido (ORCID:0000-0002-5648-066X) Jaime de Juan-Sanz (ORCID:0000-0002-1212-5623) Timothy Ryan (ORCID:0000-0003-2533-9548)
Lab:	Department of Biochemistry, Weill Cornell Medicine, New York, NY 10065, USA
Additional literature:	Data imported from deleted ticket #408 mouse Actin beta Method Using VGLUT1 Venus Knock In Mice, synaptosomes were purified using sucrose sedimentation followed by FAC sorting (titled Fluorescence Activated Synaptosome Sorting (FASS) in the paper). Samples were then separated on SDS-PAGE gels and then analyzed by LC-MS/MS. Tissue: forebrain Actin beta is localized to synaptosomal preparations @ PMID:24413018
SynGO annotation ID:	814
Dataset release (version):	20231201
View annotation as GO-CAM model: