| Annotated protein: | Actin, cytoplasmic 2 (EC 3.6.4.-) (Gamma-actin) [Cleaved into: Actin, cytoplasmic 2, N-terminally processed]. Gene symbol: ACTG1. Taxonomy: Mus musculus (Mouse). Uniprot ID: P63260 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ ACTG1 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of synaptic vesicle endocytosis (GO:1900242) |
| Synapse type(s): | hippocampus Calyx of Held Schaffer collateral synapse (CA3->CA1) |
| Annotated paper: | Wu XS, et al. "Actin Is Crucial for All Kinetically Distinguishable Forms of Endocytosis at Synapses" Neuron. 2016 Dec 7;92(5):1020-1035 PMID:27840001 |
| Figure(s): | Fig 2, 6, and 7 |
| Annotation description: | Whole cell patch recordings of P7-P10 calyxes at 22-24 oC were given a 20 ms depolarization from -80 mV to +10 mV. The initial rate of decay of the capacitance change was significantly slower (~33% of control) in the Actin, Gamma KO suggesting a slower rate of endocytosis following vesicle fusion (Fig 2. A-C). Similar results were observed when the stimulus was changed to a 20 action potential equivalent stimulus (Fig. 2 D-F), performed at 34-37 oC (Fig. 2 G) and in older mice (P13-P14) (Fig. 2 H). Hippocampal neurons were cultured from the Actg LoxP/LoxP mice transfected with Synaptophysin-pHluorin2X (SypH) with or without a Cre containing plasmid (Fig. 6 A and D). SypH can be used to study vesicle recycling as it is non-fluorescent in relatively low pH environment of vesicles but is fluorescent in the higher pH extracellular environment. Upon stimulation the decay of fluorescence was significantly slower than the control (Fig. 6 E-G). This fluorescence was quenchable by a low pH solution applied externally indicating it was not a deficit in vesicle reacidification, but instead suggesting that the vesicles had not been endocytosed (Fig. 6H). This effect was rescued by transfecting Actg in the KO cultures (Fig 7 A,B). Taken together this data suggests that Actin, Gamma is important for endocytosis. . |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Whole-cell patch clamp Optical physiology |
| Annotator(s): | Ryan J. Farrell (ORCID:0000-0003-4022-8707) Ghazaleh Ashrafi (ORCID:0000-0001-7480-0826) Camila Pulido (ORCID:0000-0002-5648-066X) Jaime de Juan-Sanz (ORCID:0000-0002-1212-5623) Timothy Ryan (ORCID:0000-0003-2533-9548) |
| Lab: | Department of Biochemistry, Weill Cornell Medicine, New York, NY 10065, USA |
| SynGO annotation ID: | 811 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |