| Annotated protein: | Voltage-dependent L-type calcium channel subunit alpha-1C (Calcium channel, L type, alpha-1 polypeptide, isoform 1, cardiac muscle) (Rat brain class C) (RBC) (Voltage-gated calcium channel subunit alpha Cav1.2). Gene symbol: CACNA1C. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P22002 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CACNA1C |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of presynaptic membrane (GO:0099056) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Tippens AL, et al. "Ultrastructural evidence for pre- and postsynaptic localization of Cav1.2 L-type Ca2+ channels in the rat hippocampus" J Comp Neurol. 2008 Feb 1;506(4):569-83 PMID:18067152 |
| Figure(s): | Fig 5 |
| Annotation description: | Developed an antibody against the pore forming subunit of Cav1.2 that was used in light and electron microscopy of fixed and stained rat brain sections. Fig. 1 shows characterization and validation of antibody specificity. Immunoreactivity against the Cav1.2 pore-forming subunit (Cav1.2-IR) was observed in both myelinated and unmyelinated axons. Within labeled axons, Cav1.2-IR was either clustered or filled the intracellular space (Fig. 5A). Axon terminals were also labeled, especially in CA1 stratum radiatum (Fig. 5B) and dentate gyrus (DG) stratum moleculare and least in CA3 stratum lucidum (Table 1). A majority of the labeled axon terminals formed asymmetric synapses. Cav1.2-IR was also observed in a large fraction of mossy fiber terminals in CA3 stratum lucidum and DG central hilus (Table 1), which generally formed asymmetric contacts with unlabeled dendrites (Fig. 5C,D). In these terminals, Cav1.2-IR formed clusters along the plasma membrane at synaptic and nonsynaptic sites but was also detected near nonsynaptic junctions with unlabeled dendrites resembling puncta adherentia. 21/6/2017 Pim - I regard the predominant intracellular localization of Cav21 in these preparation as reflecting a non-canonical localization for this protein (protein synthesis, inactive pool) and I refrain from annotation of this localization. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Ryan J. Farrell (ORCID:0000-0003-4022-8707) Ghazaleh Ashrafi (ORCID:0000-0001-7480-0826) Camila Pulido (ORCID:0000-0002-5648-066X) Jaime de Juan-Sanz (ORCID:0000-0002-1212-5623) Timothy Ryan (ORCID:0000-0003-2533-9548) |
| Lab: | Department of Biochemistry, Weill Cornell Medicine, New York, NY 10065, USA |
| SynGO annotation ID: | 809 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |