| Annotated protein: | Profilin-2 (Profilin II). Gene symbol: PFN2. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q9JJV2 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ PFN2 |
| Ontology domain: | Biological Process |
| SynGO term: | presynaptic modulation of chemical synaptic transmission (GO:0099171) |
| Synapse type(s): | striatum, glutamatergic |
| Annotated paper: | Pilo Boyl P, et al. "Profilin2 contributes to synaptic vesicle exocytosis, neuronal excitability, and novelty-seeking behavior" EMBO J. 2007 Jun 20;26(12):2991-3002 PMID:17541406 |
| Figure(s): | Fig6 and Fig7 |
| Annotation description: | Spontaneous fusion frequency is increased in slices (Fig 6D) while the amplitude of the spontaneous responses (Fig 6A) and number of synapses (Fig 6B) remain the same in the pfn2 null mutant. Increasing the extracellular calcium also produced a bigger EPSC amplitude in pfn2 KO mice (Fig6E). These data show that the vesicular release probability is increased in absence of Profilin2. This could be related to altered presynaptic actin dynamics since depolarisation-induced actin polymerisation is synaptosomes from pfn2 null mice was impaired (Fig 7C) and no direct effects on proteins level of the release machinary itself were detected (Fig 7B). |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Whole-cell patch clamp |
| Annotator(s): | Jan van Weering (ORCID:0000-0001-5259-4945) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 801 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |