Annotated protein:	P2X purinoceptor 1 (P2X1) (ATP receptor) (Purinergic receptor) (RP-2 protein). Gene symbol: P2RX1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P47824
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SynGO gene info:	SynGO data @ P2RX1
Ontology domain:	Cellular Component
SynGO term:	integral component of postsynaptic membrane (GO:0099055)
Synapse type(s):	hippocampus
Annotated paper:	Rodrigues RJ, et al. "Presynaptic P2X1-3 and alpha3-containing nicotinic receptors assemble into functionally interacting ion channels in the rat hippocampus" Neuropharmacology. 2016 Jun;105:241-257 PMID:26801076
Figure(s):	Figures 1 and 3
Annotation description:	Figure 1 shows enriched binding of P2X ligands to purified synaptosomes indicating synaptic localization. Figure 3A highlights how this synaptic associated receptor distributes across synaptic sub-compartments. Synaptosomes differentially detergent extracted to give presynaptic (pre), postsynaptic (post) and neuronal active zone (NAZ) show an enriched post-synaptic association of P2X-1 in pH 8 detergent insoluble fraction (post). Further, figure 3 shows selective staining with same P2X-1 selective antibody on intact synaptosomes immobilized on glass. (Antibodies quality controlled by Alamone). 30/10/2017 Pim - Based on the known transmembrane topology of the protein, the "integral component of ... membrane" term was chosen.
Evidence tracking, Biological System:	Intact tissue
Evidence tracking, Protein Targeting:	Antibody (detection)
Evidence tracking, Experiment Assay:	Western blot Biochemical fractionation (generic)
Annotator(s):	Noa Lipstein (ORCID:0000-0002-0755-5899) Cordelia Imig (ORCID:0000-0001-7351-8706) Vincent O'connor (ORCID:0000-0003-3185-5709) Nils Brose (ORCID:0000-0003-0938-8534)
Lab:	Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, 37075 Göttingen, Germany
SynGO annotation ID:	798
Dataset release (version):	20231201
View annotation as GO-CAM model: